摘要
目的:建立同时测定清肺抑火丸中盐酸小檗碱、盐酸黄柏碱、苦参碱含量的毛细管电泳法。方法:以盐酸伪麻黄碱为内标,采用未涂层弹性融硅石英毛细管柱(75μm×55 cm,有效长度47 cm),运行缓冲液100 mmol.L-1磷酸二氢钠溶液+40%乙醇(pH 5.75),分离电压23 kV,重力进样15 s(高度15 cm),检测波长208 nm。结果:盐酸小檗碱、盐酸黄柏碱、苦参碱分别在40.5～81.0,0.896～3.584,6.0～36.0 mg.L-1线性关系良好,回收率分别为96.0%,100.5%,97.8%,RSD均<1.85%。结论:该方法专属性强、结果准确可靠、重复性好,可用于清肺抑火丸的质量控制。
Objective： To establish a method for simultaneous determining of berberine hydrochloride,phellodendrine hydrochloride and matrine in Qingfei Yihuo pills by capillary electrophoresis.Method： With pseudoephedrine hydrochloride as the internal standard,separation was performed on a uncoated fused silica capillary of 55 cm × 75 μm（47 cm effective length）.100 mmol.L-1 NaH2PO4 ＋ 40% ethanol solution（pH 5.75） was selected as the running buffer,separation voltage was 23 kV,samples was injected by gravity in 15 s（15 cm）,detection wavelength was at 208 nm.Result： Calibration curves for berberine hydrochloride,phellodendrine hydrochloride and matrine were linear in the concentration range of 40.5-81.0,0.896-3.584,6.0-36.0 mg.L-1.The average recoveries were 96.0%,100.5%,97.8%,respectively,with RSD were not more than 1.85%.Conclusion： This method was
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第13期113-116,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省自然科学基金项目(5002841)
关键词
毛细管电泳法
清肺抑火丸
盐酸小檗碱
盐酸黄柏碱
苦参碱
capillary electrophoresis
Qingfei Yihuo pills
berberine hydrochloride
phellodendrine hydrochloride
matrine
