摘要
将从小萝卜中分离的硫堇蛋白类基因RsAFP1通过基因工程的方法导入带有泛素Ubiquitin启动子及抗除草剂Bar基因的植物双元表达载体pCAMBIA1300中,经农杆菌介导对圣稻13进行遗传转化。对获得的阳性转基因植株进行稻瘟病菌体外及室内苗期稻瘟病菌接种抗性鉴定,结合田间自然发病情况,对转基因植株的抗稻瘟病性进行综合评价。初步结果表明,外源RsAFP1基因的导入可在一定程度上提高水稻对稻瘟病的抗性。
A γ-thionin protein gene termed RsAFP1 isolated from the radish by PCR was introduced into the modified binary vector pCAMBIA1300.Six positive transformants were obtained by agrobacterium-mediated transformation to rice callus.Further,the in vitro bacterial inhibition test and indoor rice blast fungus inoculation to rice seedling were conducted.In combination with the natural incidence in field,the resistance of transgenic lines were comprehensive evaluated.The results showed that introduction of RsAFP1 gene could improve rice blast resistance.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2013年第4期335-343,共9页
Chinese Journal of Rice Science
基金
国家转基因植物新品种培育重大专项(2009ZX08001-010B)
