摘要
为了明确稻瘟病菌不同拼接版本基因组序列的差异,促进合理利用不同拼接版本基因组序列,比较了不同版本稻瘟病菌基因组数据库微卫星序列的分布情况,功能基因的位置及长度差异,SSR引物的位置差异等。结果表明,不同基因组数据库在序列长度上差异明显,功能基因的位置、SSR引物位置和SSR核苷酸重复类型数量也差异明显。无毒基因Avr-Pizt连锁标记比较分析表明Avr-Pizt基因定位在不同数据库中差别明显。不同稻瘟病菌数据库中,第7染色体的序列与其上的BAC序列之间差异显著,虽然这不影响功能基因的分析,但影响无毒基因图位克隆的定位区间的判断。因此,稻瘟病菌研究者应根据实际情况选择相应的数据库。
To promote the scientific utilization of M.oryzae databases,the location and length of functional genes,the distribution and number of SSR loci,SSR primer＇s location,and other characteristics in four M.oryzae databases and Chr7 BAC sequence database were compared.The results suggest that the length and annotated gene numbers,location of functional genes,SSR numbers of nucleotide repeats and SSR primer location in the four M.oryzae databases and Chr7 BAC sequence database differ.AvrPiz-t linked marker location assay shows that genome sequences in different databases have significant differences.Our results also indicate significant differences among the four M.oryzae databases and Chr7 BAC sequence database.Though the difference among different databases exerting not effect on the gene homolog clone,it affects the map based cloning of avirulence gene.In a word,researchers need to choose which database can be used in their studies according to the purpose.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2013年第4期425-433,共9页
Chinese Journal of Rice Science
基金
公益性行业(农业)科研专项经费资助(201203014)
国家自然科学基金资助项目(30900933
30970082
31101135)
浙江省自然科学基金资助项目(Y3110537)
浙江省重大科技专项(2012C12901-1
2012C12901-2)
关键词
稻瘟病
稻瘟病菌
基因组
图位克隆
无毒基因
rice blast
Magnaporthe oryzae
genome
map-based cloning
avirulence gene
