摘要
A novel sensing system based on fluorescence resonance energy transfer (FRET) between CdTe quantum dots (QDs) and Rhoda-mine B (RB) was established for the detection of matrix metalloproteinases (MMOL/LPs). In this system, 535-nm-emitting quantum dots (QDs) were bound to Rhodamine B (RB) via a MMOL/LP-specific peptide. A 76% reduction in luminescence was achieved because of FRET. Release of RBs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 73% rise in luminescence over 60 min. The design platform of the nanosensor is flexible and can be fine-tuned for a wide array of applications such as the detection of biomarkers, early diagnosis of disease, and monitoring therapeutic efficacy simply by changing the sequence of the peptide linker.
A novel sensing system based on fluorescence resonance energy transfer （FRET） between CdTe quantum dots （QDs） and Rhoda- mine B （RB） was established for the detection of matrix metalloproteinases （MMOL/LPs）. In this system, 535-nm-emitting quan- tum dots （QDs） were bound to Rhodamine B （RB） via a MMOL/LP-specific peptide. A 76% reduction in luminescence was achieved because of FRET. Release of RBs by peptide cleavage restores radiative QD photoluminescence. Initial studies observed a 73% rise in luminescence over 60 min. The design platform of the nanosensor is flexible and can be fine-tuned for a wide array of applications such as the detection of biomarkers, early diagnosis of disease, and monitoring therapeutic efficacy simply by changing the sequence of the peptide linker.
基金
supported by the National Natural Science Foundation of China (81000666, 81071194 and 81171395)
the Major Project of the Ministry of Science and Technology for New Drug Development (2009ZX-09310-004)
