摘要
目的通过鉴定参与白藜芦醇(RSV)诱导人急性早幼粒白血病HL-60细胞凋亡的磷酸化蛋白质,了解介导白藜芦醇诱导HL-60细胞的凋亡机制。方法以一定浓度的RSV作用HL-60细胞一定时段后,富集磷酸化蛋白质,通过双向电泳(2-DE)分离,质谱分析鉴定差异蛋白。结果以100μmol/LRSV处理HL-60细胞12h,2-DE显示同对照组比较,其磷酸化蛋白质有明显变化,经过质谱鉴定后,这些蛋白质大部分为内质网及蛋白质折叠相关蛋白。结论内质网及蛋白质折叠可能参与了RSV诱导HL-60细胞凋亡机制。
Objective To understand the mechanism of resveratrol (RSV)-induced HL60 celluar apoptosis through identifying the phosphorylated proteins involved.Methods HL60 cells were treated with RSV of a certain concentration for a certain amount of time.Enriched phosphorylated protein were separated by two-dimensional electrophoresis (2-DE) and the differential proteins were identified by mass spectrometry.Results Treated HL60 cells with 100 μmol/L RSV,2-DE showed that the phosphorylated proteins in cells had changed markedly when compared to that in control group,and the majority of the proteins were endoplasmic reticulum and protein folding-associated proteins.Conclusion Endoplasmic reticulum and protein folding may participate the mechanism of RSV-induced HL60 cellular apoptosis.
出处
《国际检验医学杂志》
CAS
2013年第11期1348-1350,共3页
International Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(81172089)
广东省大学生创新实验资助项目(杰创1056010013)
关键词
白藜芦醇
HL-60
磷酸化蛋白
内质网
凋亡
resveratrol
HL-60
phosphorylated protein
endoplasmic reticulum
apoptosis