期刊文献+

大肠埃希菌ESBLs、AmpC酶的检测及耐药特性 被引量:2

Detection of ESBLs or AmpC β -lactamase in Escherichia coli and drug resistance
下载PDF
导出
摘要 目的 分析大肠埃希菌产超广谱β-内酰胺酶(ESBLs)、头孢菌素(AmpC)酶的情况及耐药特性,指导临床合理用药.方法 收集2011年1月至2012年6月我院临床分离的大肠埃希菌287株.用双纸片确诊法检测ESBLs,三维试验检测AmpC酶,KB法进行药敏试验.结果 287株大肠埃希菌中,产ESBLs 119株(41.5%),产AmpC酶47株(16.4%),同时检出产ESBLs和AmpC酶16株(5.6%).单一产ESBLs或AmpC酶的菌株有较高的耐药性,同时检出ESBLs和AmpC酶的菌株对多种抗菌药耐药,所有菌株对亚胺培南均敏感.结论 大肠埃希菌ESBLs和AmpC酶的分离率及耐药率都较高,同时检出ESBLs和AmpC酶的菌株具多重耐药性;亚胺培南具有良好的抗菌活性.临床应加强ESBLs和AmpC酶的检测及耐药监测,采取有效措施防止耐药菌株产生. Objective To investigate extended-spectrum β-lactamases (ESBLs),AmpC β-lactamase and drug resistance in Escherichia coli (E.coli) for instructing rational medication uses.Methods 287 E.coli strains were isolated in our hospital from January 2011 to June 2012.ESBLs were detected by the double-disk method; AmpC were detected by the three dimensional extract test; and the drug sensitivity was tested by the KB method.Results Of the 287 E.coli strains,119 (41.5%) strains produced ESBLs and 47 (16.4%) strains produced AmpC,and ESBLs and AmpC were simultaneously detectable in 16 strains (accounting for 5.6%).ESBLs-or AmpC-producing strains had a higher drug resistance; ESBLs-and AmpC-producing strains had multiple drug resistance.All the strains had susceptibility to imipenem.Conclusions The rates of isolation and drug resistance are higher for ESBLs-or AmpC-producing E.coli; ESBLs-and AmpC-producing strains have multiple drug resistance.Imipenem has good antibacterial activity.Detection of ESBLs and AmpC and monitoring of drug resistance should be strengthened clinically and effective measures must be taken to prevent resistant strains.
出处 《国际医药卫生导报》 2013年第13期1893-1895,共3页 International Medicine and Health Guidance News
基金 广东省社会发展攻关课题(2009B030801284)
关键词 大肠埃希菌 超广谱Β-内酰胺酶 AMPC酶 耐药性 Escherichia coli Extended spectrum β -lactamase AmpC Drug resistance
  • 相关文献

参考文献6

二级参考文献44

共引文献43

同被引文献26

  • 1Chen T, Feng Y, Yuan JL, et al. Class 1 integrons contributes to antibiotic resistance among clinical isolates of Escherichia coli producing extended-spectrum beta-lactamases. Indian J Med Microbiol, 2013,31:385-9.
  • 2Jones RN, Castanheira M, Hu B, et al. Update of contemporary antimicrobial resistance rates across China: reference testing results for 12 medical centers (2011). Diagn Microbiol Infect Dis, 2013,77:258-66.
  • 3Silva-Sanchez J, Cruz-Trujillo E, Barrios H, et al. Characterization ofPlasmid-Mediated Quinolone Resistance (PMQR) Genes in Extended-Spectrum beta-Lactamase- Producing Enterobacteriaceae Pediatric Clinical Isolates in Mexico. PLoS One, 2013,8:e77968.
  • 4Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing ; Twentieth informational supplement[S]. CLSI document M 100-S20 Wayne. PA : Clinical and Laboratory Standards Institute, 2010, 30(1) : 1-163.
  • 5Thomson KS. Extended-spectrum-beta-lactamase, AmpC, and Carbapenemase issues. J Clin Microbiol, 2010,48(4):1019-1025.
  • 6Renvoise A, Decre D, Amarsy-Guerle R, et al. Evaluation of betaLACTA test, a rapid test detecting resistance to third-generation cephalosporins in clinical strains of Enterobacteriaceae. J Clin Microbiol, 2013,51(12):4012- 4017.
  • 7Shayan S, Bokaeian M, Shahraki S. Prevalence and Molecular Characterization of AmpC-Producing Clinical Isolates of Escherichia coli from Southeastern Iran. Microbial drug resistance (Larchmont, N.Y.), 2013.
  • 8Geyer CN, Reisbig MD, Hanson ND. Development of a TaqMan multiplex PCR assay for detection of plasmid- mediated ampC beta-lactamase genes. J Clin Microbiol, 2012,50(11):3722-3725.
  • 9Park YJ, Yu JK, Lee S, et al. Prevalence and diversity of qnr alleles in AmpC-producing Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii and Serratia marcescens: a multicentre study from Korea. J Antimicrob Chemother, 2007,60(4):868-71.
  • 10Jeong HS, Bae IK, Shin JH, et al. Prevalence of plasmid- mediated quinolone resistance and its association with extended-spectrum beta-lactamase and AmpC beta- lactamase in Enterobacteriaceae. The Korean journal of laboratory medicine, 2011,31(4):257-64.

引证文献2

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部