期刊文献+

Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy 被引量:2

Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy
原文传递
导出
摘要 A penetrating view of the three-dimensional nanostructure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscopy are quite promising for insect morphology studies and, particularly, provide an attractive alternative to the destructive techniques used for investigating internal soft tissues. A penetrating view of the three-dimensional nanostructure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscopy are quite promising for insect morphology studies and, particularly, provide an attractive alternative to the destructive techniques used for investigating internal soft tissues.
出处 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第7期410-414,共5页 中国物理B(英文版)
基金 the National Basic Research Program of China(Grant Nos.2012CB825800 and 2009CB930804) the Key Program of the National Natural Science Foundation of China(Grant Nos.10734070 and 10774144) the National Natural Science Foundation of China(Grant Nos.11205189,10979055,31071909,and 31272358) the Knowledge Innovation Program of the Chinese Academy of Sciences(Grant No.KJCX2-YW-N42)
关键词 X-ray microscopy computed tomography synchrotron radiation source morphology X-ray microscopy computed tomography synchrotron radiation source morphology
  • 相关文献

参考文献27

  • 1Hennig W 1965 Annu. Rev. Entomol. 10 97.
  • 2Zhang K, Li D, Zhu P, Yuan Q, Huang W, Liu X, Hong Y, Gao G, Ge X and Zhou H 2010 Anal. Bioanal. Chem. 397 1.
  • 3Li D, Zhang K, Zhu P, Wu Z and Zhou H 2011 Anal. Bioanal. Chem. 401 1.
  • 4Betz O, Wegst U, Weide D, Heethoff M, Helfen L, Lee W K and Cloetens P 2007 J. Microsc. 227 51.
  • 5Sakdinawat A and Attwood D 2010 Nat. Photonics 12 840.
  • 6Kaulich B, Thibault P, Gianoncelli A and Kiskinova M 2011 J. Phys. Condens. Matter 23 083002.
  • 7Withers P 2007 Mater. Today 10 26.
  • 8Zhang X Z, Xu Z, Zhen X J, Wang Y, Guo Z, Yan R, Chang R, Zhou R R and Tai R Z 2010 Acta Phys. Sin. 59 4535 (in Chinese).
  • 9Chen Y T, Chen T Y, Yi J, Chu Y S, Lee W K, Wang C L, Kempson I M, Hwu Y, Gajdosik V and Margaritondo G 2011 Opt. Lett. 36 1269.
  • 10Youn H S and Jung S W 2006 J. Microsc. 223 53.

同被引文献2

引证文献2

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部