摘要
OBJECTIVE:To study on effects of Lichong decoction on expression of apoptosis-controlling genes,Bcl-2 and Bcl-2-associated X protein(Bax) mRNAs in hysteromyoma tissue of the hysteromyoma model rat.METHODS:Fifty Wistar female rats were randomly divided into a normal group,a model group,a Lichong decoction group,a Guizifuling capsule group and a Mifepristone group.The hysteromyoma rat model was established by intraperitoneal injection of exogenous estrin and progestogens.Pathological examination of uterine tissue,uterine coefficient and uterine transverse diameter were made under optic microscope and expressions of Bcl-2 and Bax mRNAs in uterine tissue in the groups were detected with real-time fluorescent quantitative polymerase chain reaction(PCR) technique.RESULTS:After treatment,under microscope it was found that in the Lichong decoction group myometrium thinned,muscle fiber slightly overgrowth or long and thin,regular arrangement,inserting phenomenon of inner circular muscle and external longitudinal muscle was occasionally or not seen in the Lichong decoction group.The uterine coefficient and the uterine transverse diameter significantly decreased(P<0.01),and Bcl-2 mRNA expression significantly decreased(P<0.01) and Bax mRNA expression significantly increased in hysteromyoma tissue(P<0.01) in the Lichong decoction group as compared with the model group.CONCLUSION:Therapeutic effects of Lichong decoction on hysteromyoma is related with decrease of Bcl-2 mRNA expression and increase of Bax mRNA expression.
OBJECTIVE： To study on effects of Lichong decoc- tion on expression of apoptosis-controlling genes, Bcl-2 and Bcl-2-associated X protein （Bax） mRNAs in hysteromyoma tissue of the hysteromyoma model rat. METHODS： Fifty Wistar female rats were randomly divided into a normal group, a model group, a Li- chong decoction group, a Guizifuling capsule group and a Mifepristone group. The hysteromyo- ma rat model was established by intraperitoneal in- jection of exogenous estrin and progestogens. Pathological examination of uterine tissue, uterine coefficient and uterine transverse diameter were made under optic microscope and expressions of Bcl-2 and Bax mRNAs in uterine tissue in thegroups were detected with real-time fluorescent quantitative polymerase chain reaction （PCR） tech- nique. RESULTS： After treatment, under microscope it was found that in the Lichong decoction group myome- trium thinned, muscle fiber slightly overgrowth or long and thin, regular arrangement, inserting phe- nomenon of inner circular muscle and external lon- gitudinal muscle was occasionally or not seen in the Lichong decoction group. The uterine coeffi- cient and the uterine transverse diameter signifi- cantly decreased （P〈0.01）, and Bcl-2 mRNA expres- sion significantly decreased （P〈0.01） and Bax mRNA expression significantly increased in hystero- myoma tissue （P〈0.01） in the Lichong decoction group as compared with the model group. CONCLUSION： Therapeutic effects of Lichong de- coction on hysteromyoma is related with decrease of Bcl-2 mRNA expression and increase of Bax mRNA expression.
基金
Supported by Beijing City Natural Science Fund (No.7082015)
National Natural Science Fund (No. 81073096)
"Middle-young Aged Core Talent Cultural Plant" of Beijing City University Talent Strengthening Education Plant (No.PHR201008403)
