摘要
为探究谷子抗病育种的理论基础,采用Blast比对的方法,利用已知的谷子抗"拿捕净"除草剂的基因组序列,对谷子抗"拿捕净"基因进行SSR分子连锁标记筛选研究。利用抗"拿捕净"基因的已知序列找到了在谷子基因组中第7和第9条染色体上均存在同源序列,分别在这些同源序列上下游寻找SSR引物,在谷子F2群体中进行SSR标记的筛选,进行共分离分析。结果表明:位于第7染色体上的2个SSR标记SIMS13569和SIMS13512与谷子抗"拿捕净"基因性状共分离,即表现为连锁遗传,该标记可用于分子标记辅助选择,对谷子抗"拿捕净"基因育种具有重要的现实意义;而第9染色体上的序列不具有抗"拿捕净"的作用。
Utilizing the known genome sequence of herbicide-resistant to Sethoxydim in foxtail millet,selection SSR markers which was linkage of herbicide-resistant to Sethoxydim was studied.Using Blast alignment method,the homologous sequences of herbicide-resistant to Sethoxydim gene was found in the seventh and the ninth chromosomes in foxtail millet.In the upstream and downstream of homologous sequences,primers were designed to select SSR molecular markers.In the F2population of foxtail millet,the herbicide-resistant to Sethoxydim gene was linkage of two SSR markers,SIMS13569and SIMS13512which were in the seventh chromosome,and could be used for molecular marker assisted selection.It is important to the genetic breeding of herbicide-resistant to Sethoxydim in foxtail millet.However,the homologous sequence in the ninth chromosome was not own the effect of herbicide-resistant to Sethoxydim.
出处
《黑龙江农业科学》
2013年第7期5-7,59,共4页
Heilongjiang Agricultural Sciences
基金
国家现代农业产业技术体系专项资金资助项目(CARS-07)
黑龙江省农业科技创新工程资助项目
