摘要
目的体外检测三氧化二砷(As2O3)对人恶性纤维母细胞株MG-63(骨肉瘤细胞)及人正常纤维母细胞株MRC-5增殖的浓度效应,评价As2O3对恶性纤维母细胞毒作用的强度及特异性。方法应用噻唑蓝(MTT)法检测5 d内0.25～8μmol/L浓度的As2O3对细胞株MG-63及MRC-5增殖的抑制率。以累积正态分布函数拟合二者的浓度-抑制率曲线,比较50%抑制浓度(IC50)。以正态分布函数拟合二者抑制率的差值,推断最大差值及对应浓度的95%可信区间。结果1μmol/L浓度的As2O3对MG-63细胞抑制率第5天达58.8%,对MRC-5细胞最高仅达27.6%,8μmol/L浓度的As2O3对MRC-5细胞的抑制率达55.9%。使用第4天的浓度及抑制率分别拟合MG-63与MRC-5细胞剂量效应曲线的累积正态分布函数,决定系数(r2)值均大于0.98,IC50分别为0.968 6μmol/L和6.103 8μmol/L,其比值为6.30。拟合MG-63及MRC-5细胞增殖抑制率差值的正态分布函数,r2=0.990 7,最大抑制率差值为25.36%,95%可信区间对应As2O3的浓度为0.487 5μmol/L和1.049 4μmol/L。结论As2O3可有效抑制人恶性纤维母细胞增殖,在特定浓度区域内具有一定的特异性(安全性)。
Objective To examine in vitro the concentration effect of arsenic trioxide（As2O3） on the proliferation of human malignant fibroblast cell line MG-63（osteosarcoma） and human normal fibroblast cell line MRC-5,and to evaluate its intensity and the specificity on the malignant fibroblast cell cytotoxicity action.Methods The proliferation-inhibition rate of MG-63 and MRC-5 cell lines treated with As2O3（0.25-8 μmol/L） were observed within 5 d by the methy thiazoloyl tetrazolium（MTT） assay.The accumulation normal distribution function was adopted to fit their concentration-inhibition rate curves and the 50%of inhibition concentrations（IC50） were compared.To fit the differences of concentration-inhibition rates between MG-63 and MRC-5 with the normal distribution function and to deduce the maximum difference and the 95%credibility interval of corresponding concentration of As2O3.Results The maximum inhibition rate of MG-63 cells treat with 1 μmol/L As2O3 was 58.8%on 5 d and which of MRC-5 cells only reached 27.6%;the inhibition rate of MRC-5 with 8 μmol/L As2O3 reached 55.9%.To fit the accumulative normal distribution function of the dose-effect curves of MG-63 and MRC-5 cells with the concentration-inhibition rate on 4 d,the determination coefficients（r2） all were more than 0.98,IC50 was 0.968 6 and 6.103 8 μmol/L respectively,and their ratio was 6.30.To fit the normal distribution function of the differences of concentration-inhibition rates between MG-63 and MRC-5 on 4 d（r2=0.990 7）,the maximum difference of inhibition rates was 25.36%and the orresponding concentration of As2O3 in the 95%credibility interval was 0.487 5 μmol/L,1.049 4 μmol/L respectively.Conclusion As2O3 can effectively inhibit the proliferation of human malignant fibroblast cell（osteosarcoma） with certain specificity in the specific concentration interval.
出处
《中国药业》
CAS
2013年第13期12-14,共3页
China Pharmaceuticals
