摘要
目的:检测PTP4A1基因在舌鳞癌组织及舌鳞癌细胞系TCA8113中的表达,并探讨其表达水平与舌鳞癌临床病理参数的关系。方法:应用免疫组化SP法检测30例舌鳞癌和15例正常舌组织石蜡包埋组织中PTP4A1的表达;对15例新鲜舌鳞癌组织进行real-time PCR,检测PTP4A1在舌鳞癌组织、癌旁组织、正常舌组织的表达,此外对TCA8113细胞中PTP4A1的表达水平也进行了检测。结果:免疫组化结果显示,PTP4A1表达于舌鳞癌组织的细胞质和细胞核,在舌鳞癌组织中呈阳性和强阳性表达,而在正常舌组织中呈阴性和弱阳性表达;PTP4A1在舌鳞癌的阳性表达率为83%,显著高于其在正常舌组织中的表达率33.3%;PTP4A1在舌鳞癌组织和正常舌组织的表达差异有统计学意义(P=0.001<0.05);而且PTP4A1的表达水平与肿瘤的淋巴转移相关(P=0.014<0.05),而与病人的性别、年龄、肿瘤的大小、肿瘤的分期分级无明显相关。Real-time PCR实验结果显示舌鳞癌组织及舌鳞癌细胞系TCA8113细胞中的PTP4A1的mRNA表达水平高于其在癌旁和正常舌组织的表达,癌旁组织的表达高于正常舌组织的表达(P=0.000<0.05)。结论:PTP4A1基因mRNA水平和蛋白水平表达在舌鳞癌组织中表达均较癌旁和正常舌组织高,可能在舌鳞癌的发生发展过程中起着一定作用。
Objective:To investigate PTP4A1 gene expressions in human tongue squamous cancer tissues and TCA8113 cell line and to explore the relationship between PTP4A1 expressions and clinicopathological features of human tongue squamous cancer.Methods:Expressions of PTP4A1 in 30 cases of human tongue squamous cell carcinoma and 15 cases of normal tongue mucosa paraffin-embedded specimens were examined by immunohistochemical technology and in 15 freshly-taken tongue squamous tissues and TCA8113 cells by real-time PCR.Results:Immunoreactive staining was located in the cytoplasm and cell nucleus of human tongue squamous cancer.Positive rate of PTP4A1 in human tongue squamous cancer was 83%,which was remarkably higher than that in normal tongue tissue 33.3%(P=0.001<0.05),with significant differences.Expression of PTP4A1 was closely related with lymphatic metastases(P=0.014<0.05),but had no relationship with age,gender,tumor size,and clinical stage.Result of real-time PCR also showed that PTP4A1 mRNA level was higher in human tongue squamous cancer tissues and TCA8113 cells than in para-carcinoma and normal tissues(P=0.000<0.05).Conclusions:Both PTP4A1 mRNA and protein expressions are higher in human tongue squamous cancer tissues than in para-carcinoma and normal tongue tissues.PTP4A1 expression may play a significant role in human tongue squamous cancer development and metastases.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2013年第8期854-858,共5页
Journal of Chongqing Medical University
