摘要
【目的】用TritonX-100(TX)诱导一种嗅觉障碍模型,观察不同时点嗅上皮的组织学改变和小鼠嗅觉功能的变化,并对两者进行相关性分析。【方法】用0.7%的TX对8～10周的BALB/C小鼠灌鼻以诱导小鼠嗅觉障碍,分别于灌鼻后第3、7、21、49、56天行觅食实验,以觅食时间的长短评估小鼠嗅觉功能的改变;用免疫组织化学染色(IHC)的方法,检测小鼠嗅上皮(OE)中成熟嗅神经元(ORN)数量、嗅上皮厚度、嗅上皮细胞总数的变化;并对小鼠觅食时间的改变和嗅上皮中成熟嗅神经元数量的变化行相关性分析。【结果】TX诱导小鼠嗅觉障碍后,第3～7天时,小鼠嗅上皮中成熟ORN的数量急剧下降,嗅上皮的厚度变薄,细胞总数明显减少,小鼠觅食时间显著延长。第21天时,嗅上皮中成熟ORN开始增多,嗅上皮厚度开始恢复,嗅上皮中有大量新生细胞形成,以嗅觉标记蛋白(OMP)阴性细胞为主。此时小鼠嗅觉功能部分恢复,但觅食时间仍长于对照组。第49～56天时,小鼠嗅上皮中ORN数量、嗅上皮厚度、细胞总数及觅食时间恢复至正常水平。小鼠觅食时间与其嗅上皮中成熟ORN数量具有明显的相关性(r=-0.757,P<0.001)。【结论】联合运用行为学及组织化学的方法,可有效地评估小鼠嗅觉功能;用TX可成功诱导小鼠嗅觉障碍模型,该模型简便、稳定、可靠,具有可恢复的特点。
[Objective] To investigate a mice model of dysosmia induced with TritonX-100 and observe both the histological changes of olfactory epithelium and functional abnormalities of mouse olfactory organ.The above relationship between the structure and function is evaluated in the study.[Methods] The dysosmia model was established using the mice of 8 to 10-week-old induced through intranasal irrigation with 0.7% Triton X-100（TX）.The The ＂buried food pellet＂ test was conducted separately at 3,7,21,49,and 56 days post irrigation to evaluate the mouse olfactory functional abnormalities,combined with the means of olfactory marker protein （OMP） immunohistochemical analysis of olfactory epithelia （OE） for identification of the changes in mature olfacory receptor neurons （ORN）,total cells and thickness of OE.And the relationship between ＂buried food pellet＂ test outcome and the mature ORN population of OE was detected.[Results] 3 to 7 days after intranasal irrigation with TritonX-100,the mature ORN in olfactory epithelium,OE thickness and total OE cells were distinctly reduced.And the average time for finding food was obviously extended.At 21 days post-irrigation,the mature ORN in olfactory epithelium began to increase,and meanwhile the OE thickness recovered.A large number of newly regenerated cells were observed,most of which are OMP（-） cells.The olfactory function partly recovered,but the average time spent to find food is still longer than the control group.At 49 to 56 days post-irrigation,the mature ORN cells,average thickness,total OE cells restored and the ＂buried food pellet＂ test outcome recovered to normal.The food-finding time of mice was significantly correlated with the number of mature ORN in OE （r =-0.757,P 〈 0.001）.[Conclusions] Behaviour test,combined with morphology and histochemistry analysis used in this experiment can be useful in assessing odor detecting capability.The dysosmia model was induced successfully by intranasal irrigation with 0.7% Triton X-100 （TX）,which was convenient,stable,reliable and restorable.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2013年第3期371-377,共7页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省科技计划项目(2009B030801202)
