摘要
目的:研究芒果苷(Mangiferin,MF)对α-葡萄糖苷酶的抑制作用。方法:通过建立体外α-葡萄糖苷酶抑制剂微孔筛选模型,对芒果苷进行α-葡萄糖苷酶抑制活性研究,并采用林贝氏作图法判断抑制作用类型;研究芒果苷对正常小鼠可溶性淀粉、蔗糖和葡萄糖耐量的影响。结果:芒果苷(IC50=164.16 mg/L)体外α-葡萄糖苷酶抑制活性高于阳性药阿卡波糖(Acarbose,IC50=1095.65 mg/L),且抑制率随浓度增加而增加,为竞争性抑制剂;体内研究表明灌胃给予芒果苷(50mg/kg和100mg/kg)能明显抑制正常动物对淀粉、蔗糖等多糖的吸收,但对葡萄糖的吸收并无影响。结论:芒果苷有α-葡萄糖苷酶抑制活性。
He Rong,Feng Guohua,Zhang Fudong,Xiong Fan,Li Ling,Gao Lihui（Biomedical Engineering Reaserach Center,Kunming Medical University,Kunming 650500）
Objective：Mangiferin,a xanthone glucoside,is an active phytochemical exist in various plants.In the present study,the inhibitory action of mangiferin on the activity of alpha-glucosidase is investigated.Methods： An optimized microplate-based method was developed to characterize the mangiferin on the activity of α-glucosidase inhibition.The related inhibition type of mangiferin was identified from Lineweaver-Burk plots.The inhibitory effects of mangiferin on polysaccharide absorption were investigated in normal mice.Results： In vitro,mangiferin significantly inhibited the activity of α-glucosidase,in a concentration-dependent manner,with a IC50 value of 164.16 mg/L,which was more potent than that of acarbose（IC50 = 1095.65 mg/L）.The inhibition type of mangiferin was competitive.In vivo,mangiferin,given by intragastrically 50 mg/kg and 100 mg/kg,markedly reduced the absorption of starch and sucrose in normal mice,but has no effect on the absorption of glucose.Conclusion： Mangiferin has obvious inhibitory activity on α-glucosidase in vitro and in vivo.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第3期54-57,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助项目(30760306)
云南省应用基础研究计划重点项目(2006C009Z)
关键词
芒果苷
Α-葡萄糖苷酶
抑制活性
抑制类型
糖吸收
mangiferin（芒果苷） α-glucosidase inhibitory activity inhibitory type absorption
