摘要
目的:探讨氯化腺苷对腹主动脉缩窄(AAC)致大鼠心肌肥厚时TLR4及其介导的信号通路的作用。方法:大鼠采用结扎腹主动脉的方法制备AAC模型,1周后给予大鼠2-氯化腺苷0.3、0.6、1.2 mg/kg,每天一次,共12周;计算心脏质量指数(HMI)和左心室质量指数(LVMI);测定左心室收缩压(LVSP)、左心室舒张末压(LVEDP)和左心室内压最大升降速率(±dp/dtmax);采用逆转录-聚合酶链反应(RT-PCR)测定心肌心钠素mRNA(ANP mRNA)表达;ELISA法检测心肌组织TNF-α、IL-1β的含量;Western blot法检测心肌组织IκBα、TLR4的蛋白含量。结果:与假手术组相比,AAC模型组的大鼠,IκBα蛋白量明显降低,其他指标均显著增高;同模型组相比,2-氯化腺苷1.2 mg/kg组的HMI,LVMI,LVSP,LVEDP和±dp/dtmax均显著降低;2-氯化腺苷0.6、1.2mg/kg组的ANP mRNA表达和TLR4蛋白量明显下降,IκBα蛋白量明显升高;2-氯化腺苷0.3、0.6、1.2 mg/kg组心肌组织TNF-α、IL-1β的含量均显著减少。结论:氯化腺苷可以有效抑制AAC大鼠心肌肥厚,抑制炎症因子含量的增加,其作用机制可能同TLR4及其介导的NF-κB通路有关。
Objective:To explore the effect of adenosine on TLR4 and mediated signal path induced by abdominal aorta constriction in rats.Methods: Myocardial hypertrophy model in SD rats was established by adopting abdominal aorta constriction(AAC).2-CADO0.3,0.6,1.2 mg/kg were given to the rats for 11 weeks;Heart mass index(HMI) and left ventricular mass index(LVMI) were detected;Left ventricular systolic pressure(LVSP);left ventricular end-diastolic pressure(LVEDP),and maximum rate of rise/decrease of LVSP(± dp/dtmax) were detected;mRNA expression of heart atrial natriuretic peptide(ANP) was observed by RT-RCR;the expression of TNF-α,IL-1β was determined by ELISA;the expression of IκBα and TLR4 were measured by Western.Results: Compared with sham group,IκBα significantly decreased and other indicators significantly elevated in all index in AAC group;Compared with AAC group,2-CADO 1.2 mg/kg decreased HMI,LVMI,LVSP,LVEDP and ± dp/dtmax.;2-CADO 0.6,1.2 mg/kg could also remarkably down-regualte the overexpressions of ANP mRNA and TLR4 induced by AAC and significantly improved IκBα level;TNF-α and IL-1β contents effectively decreased by 2-CADO 0.3,0.6,1.2 mg/kg.Conclusion: ADO can inhibit the increased content of inflammation factor in AAC rats and has a protective effect on protects the hypertrophic myocardium,which is partially via attenuating the expression of TLR4/NF-κB.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第3期57-60,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
辽宁省科技计划项目(2009225010-40)
辽宁省教育厅创新团队项目(2009T064)
关键词
腺苷
心肌肥厚
腹主动脉缩窄
心钠素
炎症因子
adenosine(腺苷) myocardial hypertrophy abdominal aorta construction atrial natriuretic peptide inflammation factor