摘要
目的:观察抗氧化剂桑黄粗多糖对被动吸烟对小鼠肝脏氧化应激指标的影响,探讨桑黄粗多糖对被动吸烟大鼠肝脏保护作用的可能机制。方法:1月龄雄性昆明小鼠28只,适应性饲养一周后随机分为补药被动吸烟组(A)、补药组(B)、被动吸烟组(C)、对照组(D)。2周后摘眼球处死即刻提取肝脏进行SOD、CAT、GSH、GSH-PX酶活性检测。结果:四组中B组的CAT酶活性明显高于D组(P<0.05),其余组次酶活性变化明显,尤其是B组显著高于其他组次,与C组相较时差异非常显著(P<0.01);A组中GSH酶活性明显低于D组(P<0.05);B、C组SOD酶活性差异明显(P<0.05);GSH-PX酶活性四组间没有显著性差异。结论:桑黄粗多糖可以增强抗氧化酶活性,提高机体对自由基清除能力,减少肝脏氧化应激损伤程度。
Objective： to observe the effect on oxidative stress indicators of passive smoking mice liver by antioxidant mulberry yellow coarse polysaccharide, discuss mulberry yellow coarse polysaccharide on the passive smoking rat liver protective effect of the possible mechanism. Methods： 1 month 28 male kunming mice, adaptive breeding were randomly divided into a week after the passive smoking group （A）, tonic the passive smoking group （B） （C） （D） and the control group. Two weeks after picking eyeball executed immediately to extract liver SOD, CAT, GSH and GSH-PX enzyme activity detection. Results： four out of the CAT enzyme activity of group B was obviously higher than that of group D （P 〈 0.05）, and the rest of the enzyme activity change significantly, especially in B group is significantly higher than other times, a very significant difference when compared with group C （P 〈 0.01）; GSH enzyme activity in group A was lower than that in group D （P 〈 0.05）; SOD enzyme activity difference between B and C group was obviously （P 〈 0.05）; Gsh-px activity is no significant difference between four groups. Conclusion： mulberry yellow and its extract can enhance antioxidant enzymes activity of radical scavenging ability; reduce the damage for body liver oxidative stress.
出处
《体育科技》
2013年第3期71-73,共3页
Sport Science and Technology
关键词
桑黄粗多糖
被动吸烟
氧化应激
抗氧化酶
mulberry yellow coarse polysaccharide
passive smoking
oxidative stress
antioxidant enzymes
