摘要
目的探讨慢性HBV感染者甘露糖结合蛋白(MBP)基因多态性对慢性乙肝患者疾病进展的影响及与肝功能、乙肝标志物的关系。方法采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法和酶联免疫吸附试验(ELISA)对244例慢性乙肝患者(CHB)、151例肝硬化患者(LC)和88名正常对照者的MBP基因第54号密码子多态性和血清肝功能、乙肝标志物进行检测。结果 CHB轻、中度组患者MBP基因GGC/GAC基因型频率和GAC等位基因频率与对照组比较差异无统计学意义(P>0.05);CHB重度组、代偿期LC组、失代偿期LC组MBP基因GGC/GAC基因型频率和GAC等位基因频率均高于对照组(P<0.05),其中失代偿性LC组突变率最高,为36.5%;慢性HBV感染者MBP基因54号密码子突变与血清肝功能和乙肝标志物比较差异均无统计学意义(P>0.05)。结论 MBP基因第54号密码子突变与肝功能、乙肝标志物模式无明显关系,而与慢性HBV感染进展有关。
Objective：To determine the influences of Mannose binding protein（MBP） gene polymorphisms on the Liver function or HBV markers and on progression of liver disease in patients with chronic HBV infection.Method：The cordons on 54 MBP gene polymorphisms and the Liver function or HBV markers in a cohort of 395 patients with chronic HBV infection,including 244 with chronic hepatitis B（CHB）,151 with liver cirrhosis（LC） and 88 normal controls were examined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and Elisa method.Result：The MBP genotype frequencies of GGC/GAC and alleles genetic frequencies of GAC in CHB group showed no significant differences comparing to the control group（P0.05）.The MBP genotype frequencies of GGC/GAC and alleles genetic frequencies of GAC on CHB group（severe）,compensation phase of LC group and decompensation phase of LC group were higher than those in the normal control group（P0.05）,the genetic polymorphism of decompensation of LC was 36.5%,highest of all.The MBP genotype frequencies of GGC/GAC and alleles genetic frequencies of GAC of patients with chronic HBV infection were not changed with the differences of the Liver function or HBV markers.Conclusion：The codes on 54 MBP gene polymorphisms is not closely related to the Liver function or HBV marker patterns,but was associated with the progression of hepatitis B infection.
出处
《中国优生与遗传杂志》
2013年第6期31-33,共3页
Chinese Journal of Birth Health & Heredity
基金
福建省漳州市科技计划资助项目(Z2010085)
关键词
甘露糖结合蛋白
肝炎
乙型
慢性
基因
肝功能
乙肝标志物
Mannose-binding protein
Hepatitis B
chronic
Genetic polymorphism
Liver function
HBV markers
