摘要
以总酚吸附率为指标,筛选吸附纯化汉麻籽壳抗氧化多酚的最适大孔树脂。通过静态和动态吸附试验,研究吸附机理及特性;通过体外自由基清除实验和高效液相色谱(HPLC)法评价大孔树脂吸附纯化的效果。试验结果表明:HPD-600对汉麻籽壳抗氧化多酚的吸附能力最强,吸附过程符合准一级动力学模型,受液膜扩散控制;等温吸附过程符合Langmuir模型和Freundlich模型。吸附过程是放热过程,温度升高,不利于吸附。进样浓度对总酚漏出率影响较大,浓度越高,漏出率越高。进样流速对总酚漏出率影响不显著。采用梯度洗脱时,60%乙醇洗脱物的抗氧化能力和得率都最高,为目标产物。纯化后的产物相比纯化前对DPPH.、OH.和O.3种自由基的清除效果都显著提高,而且杂质含量明显减少,活性成分N-反式咖啡酰酪胺和大麻木脂素酰胺B的含量显著增加,说明利用HPD-600大孔树脂吸附纯化汉麻籽壳抗氧化多酚效果明显,方法可行。
The adsorption properties of 11 kinds of macroporous resins to hempseed hull antioxidant polyphenol were evaluated.It was found that HPD-600 had the higher adsorption ability than others.The results of static adsorption kinetics and thermodynamics of HPD-600 indicated that adsorption processing fitted the pseudo-first-order kinetics model and Langmuir and Freundlich models.The adsorption was an exothermic process so that high temperature was negative factor.The leaking ratio of total phenol during dynamic adsorption was significantly affected by concentration of sample solution other than flow rate.Gradient elution was employed in dynamic desorption process and eluate of 60% ethanol was target extract with the higher antioxidant activity and yield than others.The purified extract by HPD-600 resin had the stronger capacities in scavenging DPPH.,OH.and O 2.radicals and the less content of impurity than crude sample.The HPLC measurement found that the content of N-trans-caffeoyltyramine and Cannabisin B increased significantly after purification.The results showed that HPD-600 macroporous resin was suitable for purification of hempseed hull antioxidant polyphenol.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2013年第6期77-87,共11页
Journal of Chinese Institute Of Food Science and Technology
关键词
汉麻籽壳
多酚
抗氧化
大孔树脂
吸附动力学
hempseed hull
polyphenol
antioxidant
macroporous resin
adsorption kinetics
