摘要
目的利用金标法和酶联免疫法检测HIV的抗体,并对检测的结果进行比较分析。方法选取1780例艾滋病防治科自愿咨询者和高危人群的血液标本,在治疗的同时采用金标法和酶联免疫法两种检测方法检测患者的血液HIV抗体。结果金标法筛选检测出25例阳性患者,酶联免疫法检测出15例阳性患者。对金标法和酶联免疫法筛选的阳性率和假阳性率进行统计学分析,差异均有统计学意义(P<0.05)。结论在进行HIV抗体的检测时,金标法和酶联免疫法各自具有不同的特点,因而在实际临床使用时,需要根据不同情况选择恰当的检测方法。
Objective To utilize the methods of the Dot immunogold filtration assay and enzyme-linked immunosorbent assay for the detection of HIV antibodies, then analysis the results. Methods 1780 blood samples of voluntary counseling and high-risk groups were collected by the AIDS Prevention and Control Division of our unit, and then analyzed the HIV antibodies by using the methods of Dot immunogold filtration assay and enzyme-linked immunosorbent assay. Results 25 positive specimens were screened by the method of dot immunogold filtration assay, while 15 were screened by the method of enzyme-linked immunosorbent assay. The positive rate and false positive rate of dot immunogold filtration assay and enzyme-linked immunosorbent assay were analyzed in statistically, the differences were statistically significant （P〈0.05）. Conclusion The dot immunogold filtration assay and enzyme-linked immunosorbent assay have their different characteristics, and thus in clinical, it is important to select an appropriate method to detect HIV according to different situations.
出处
《当代医学》
2013年第20期69-70,共2页
Contemporary Medicine
关键词
HIV
金标法
酶联免疫法
临床检测
HIV
Dot immunogold filtration assay
Enzyme-linked immunosorbent assay
Clinical detection
