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三氧化二砷对MDA-MB-231细胞增殖、凋亡的影响 被引量:2

Effect of arsenic trioxide on proliferation and apoptosis of MDA-MB-231 cells
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摘要 目的观察不同浓度的三氧化二砷对三阴性乳腺癌MDA-MB-231细胞增殖、凋亡的影响。方法噻唑蓝(MTT)比色法检测不同浓度(0.2、0.4、0.6、0.8、1.0μmol.L-1)的三氧化二砷作用于三阴性乳腺癌MDA-MB-231细胞24、48、72 h后的细胞增殖率,Western blot检测不同浓度的三氧化二砷作用于MDA-MB-231细胞24 h的PCNA水平。结果不同浓度的三氧化二砷作用于三阴性乳腺癌MDA-MB-231细胞24、48、72 h后,随着时间的推移和浓度的升高,细胞增殖抑制率增加,有浓度和时间依赖性,以1.0μmol.L-1三氧化二砷作用最明显,Western blot检测显示,与对照组比较,随着浓度的升高,PCNA蛋白降低,其中以1.0μmol.L-1三氧化二砷作用后最明显。结论三氧化二砷能促进MDA-MB-231细胞的凋亡,其机制可能与下调PCNA表达的促凋亡途径有关。 Objective To observe different concentrations of arsenic trioxide on the proliferation and apoptosis of the triple negative breast cancer MDA-MB-231 cells. Methods The growth inhibition of different concentrations ( 0.2 0.4,0.6,0.8,1.0 p, mol · L -1 ) of arsenic trioxide on triple negative breast cancer MDA-MB -231 cells for 24,48,72 h was detected by means of MTT assay. The protein expression of PCNA was detected by Western blotting. Results When the MDA-MB-231 cells were treated with different concentrations of arsenic trioxide for 24 h,48 h ,72 h,with the increase of time and concentration,cell proliferation inhibition rate both increased,mean- while 1.0 txmol ~ L-1 was most obvious, besides ,western blot detection showed that, compared with control group, the expression of PC- NA protein decreased with the increase of the concentration and 1.0 μmol · L -l was most obvious as well. Conclusion Arsenic trioxide can promote apoptosis of MDA-MB-231 cells,which may be related with the decrease of PCNA expression in pro-apoptotie pathway.
出处 《安徽医药》 CAS 2013年第6期1038-1039,共2页 Anhui Medical and Pharmaceutical Journal
关键词 三阴性乳腺癌 三氧化二砷 PCNA triple negative breast cancer arsenic trioxide PCNA
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  • 1于志勇,宋丽华,卓培英,王永胜,薛兴奎,王圣芳,魏玲,张翠珍,吕宝玉,李祖国,吴泰璜.乳腺癌患者外周血hMAM测定的临床意义[J].中国现代普通外科进展,2004,7(4):235-237. 被引量:3
  • 2胡卫东,林从尧,熊斌,李春光,胡名柏.腋淋巴结阴性乳腺癌外周血hMAM检测意义[J].中国肿瘤临床,2005,32(12):674-677. 被引量:6
  • 3Bauer KR,Brown M,Cress RD,et al.Descriptive analysis of estrogen receptor(ER)negative,progesterone receptor(PR)negative,and HER-2 negative invasive breast cancer,the so-called triple-negative phenotype:a population-based study from the California cancer Registry[J].Cancer,2007,109(9):1721.
  • 4Yi S,Uhm J,Cho E,et al.Clinical outcomes of metastatic breast cancer patients with triple-negative phenotype who received platinum-containing chemotherapy[J].J Clin Oncol,2008,26:1008.
  • 5Miller K,Wang M,Gralow J,et al.Paclitaxel plus bevacizumab versus paclitaxel alone for metastatic breast cancer[J].N Engl J Med,2007,357(26):2666-76.
  • 6Zelek L,Barthier S,Riofrio M,et al.Weekly vinorelbine is an effective palliative regimen after failure with anthracyclines and taxanes in metastatic breast carcinoma[J].Cancer,2001,92(9):2267-72.
  • 7Decatris MP,Sunder S,O Byrne KJ,et al.Platinum-based chemotherapy in metastatic breast cancer:current status[J].Cancer Trert Rev,2004,30(1):53-81.
  • 8McPherson K,Steel CM,Dixon JM.ABC of breast diseases:breast cancer epidemiology,risk factors and genetics[J].Br Med J,2000,321:624-8.
  • 9Watson MA,Dintzis S,Darrow CM,et al.Mammaglobin expression in primary,metastatic,and occult breast cancer[J].Cancer Res,1999,59(13):3028-31.
  • 10Xenidis N,Markos V,Apostolaki S,et al.Clinical relevance of circulating CK-19 mRNA-positive cells detected during the adjuvant tamoxifen treatment in patients with early breast cancer[J].Ann Oncol,2007,18(10):1623-31.

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