摘要
为了研究丹参中特有的NAC转录子在丹参生长发育、激素调节和抗逆胁迫应答调节中的功能,对丹参NAC转录因子进行了克隆和分析。根据丹参毛状根cDNA文库中筛选到的NAC EST序列,克隆了丹参SmNAC1的cDNA全长序列。生物信息学分析显示基因的开放阅读框591 bp,编码166个氨基酸,相对分子质量21.66 kDa,等电点4.36 Genbank kF006346。SmNAC1蛋白的N-端具有保守的NAC_AB结构域,C-端高度变异。根据软件预测SmNAC1可能定位在细胞核。qRT-PCR分析YE+Ag+处理后SmNAC1在丹参毛状根中的表达变化,处理后2 h表达量上调至对照的1.5倍,4～12 h保持2倍的表达量,36 h时下降至对照水平以下,推测SmNAC1可能参与了丹参毛状根对YE+Ag+的胁迫应答调节。
In order to study function of NAC transcription in development, hormone regulation and the stress response of Salvia miltiorrhiza, the NAC transcription was cloned and analyzed. By retrieving cDNA database of S. miltiorrhiza hairy root one NAC uni- gene was found, then a full length of cDNA was cloned by designing specific primers and PCR amplifying. Using ORF finder it was found that the cDNA containing a NAC-AB conserved domain in N-terminal, so the cDNA was a NAC transcription factor, named as SmNACI （kF006346）. Bioinformatics analysis showed that SmNAC1 had an open reading frame （ORF） of 591 bp encoding 196 amino acids. The calculated protein had isoelectric point （pI） of 4.36 with molecular weight about 21.66 kDa. The transcription level of SmNAC1 after dealing with yeast extract （YE） and silver ion （Ag ＋ ） in S. rniltiorrhiza hairy mot was markedly stimulated up regula- ting. It was 1.4 fold compared with the control after induction 2 h, and maintained 2.0 fold on 4-12 h after induction. SmNAC1 may participate in regulation of stress response of YE ＋ Ag ＋.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第13期2063-2067,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81173490
81130070)
关键词
丹参
NAC转录因子
SmBF3—2
分子克隆
生物信息学
Salvia miltiorrhiza
basic transcription factor
stress responsiveness
bioinformatics analysis
