摘要
目的:探讨复方大七气汤对小鼠上皮性卵巢癌的治疗效果。方法:将人卵巢癌细胞株SKOV-3注射在60只雌性BALB/C小鼠右臀部皮下建立上皮性卵巢癌模型,3周后,其中52只皮下发现包块,处死其中4只行病理组织学检查以验证其造模成功,然后将模型小鼠分为模型对照组,复方红豆杉胶囊组,复方大七气汤高剂量组、复方大七气汤中剂量组、复方大七气汤低剂量组。治疗3周末处死所有小鼠,测量癌包块体积、对癌包块进行病理切片组织学检查,同时以ELISA法检测血清糖类抗原125(CA-125)、人附睾上皮分泌蛋白4(HE4)、以SP法检测癌组织增殖细胞核抗体(PCNA)、血管内皮生长因子(VEGF)、基质金属蛋白酶-9(MMP-9)及累计光密度值。结果:复方大七气汤中剂量组卵巢癌包块体积[(18.60±0.96)mm3]、血清CA125[(6.13±0.04)U.mL-1]、HE4[(16.90±0.87)pmol.L-1]、癌组织PCNA(12.1±0.56)、VEGF(10.00±0.66)、MMP-9(14.10±0.73)和累计光密度值明显低于模型对照组与复方红豆杉胶囊治疗组(P<0.01)。结论:复方大七气汤可通过抑制细胞增殖与血管生成而对上皮性卵巢癌有治疗作用。
OBJECTIVE To determine the efficiency of compound Daqiqi decoction in the treatment of epithelial ovarian canc- er model. METHODS The human ovarian cancer cell line SKOV3 was injected into 60 female BALB/c mice right gluteal sub cutaneously to establish epithelial ovarian cancer model. After 3 weeks, 52 mice were found with the subcutaneous bag pieces, 4 of them were used for model identification. The rest 48 ovarian cancer mice were further divided into model group （n = 8）, compound Hongdoushan capsules group（n- 10）, high dosed compound Daqiqi decoction group（n = 10）, middle dosed com- pound I）aqiqi decoction group（n = 10）, low dosed compound Daqiqi decoction group（n = 10） At the end of treatment, all the mice were killed. The cancer mass volume was measured and the tissues were taken for pathological changes examination. The serum levels of CA125, HFA and the cancer tissue levels of PCNA, VEGF, MMP-9, cumulative optical density value in the mice of all groups were measured and compared. RESULTS Middle dose compound Daqiqi decoction group caused obvious de- crease in cancer mass volumeE （ 18. 60±0. 96） mm3 ], serum levels of CA125 E （6. 13 ± 0. 04） U. mL1 ], HE4[ （ 16. 9{） ＋ 0. 87） pmol.1. ~2, the cancer tissue PCNA（ 12. 1 ± 0. 56） ,VEGF（ 10. 00 ±0. 66）, MMP-9（ 14. 10± 0. 73）, cumulative optical density value when compared with those of model group and compound Hongdoushan capsules group（P〈0. 01）. CONCLUSION Com- pound daqiqi decoction exerts curative effect on epithelial ovarian cancer in mice.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2013年第14期1129-1134,共6页
Chinese Journal of Hospital Pharmacy
基金
重庆市自然科学基金(编号:CSTC
2008BB5217)
