摘要
N基因起源于烟草野生种粘毛烟草(Nicotiana glutinosa),属于TIR-NBS-LRR类抗病基因,介导烟草花叶病毒(tobacco mosaic virus,TMV)的抗性,通过转座子标签法得到克隆,目前,N-TMV互作是研究最早且最多的植物-病原菌互作模型之一。笔者从转录产物、表达特征、温度敏感性、对应无毒基因等研究内容回顾了N基因在结构、表达、作用机理等方面的研究进展及现状,归纳了以N基因为TMV抗源在烤烟遗传育种中的应用及取得的成果,并从抗TMV机制研究、抗TMV种质鉴定、种质资源利用等方面对N基因在烤烟抗TMV育种中的高效利用提出了展望。
The N gene, cloned from wild tobacco （Nicotiana glutinosa）, is a type member of the Toll-IL-1 receptor homology region （TIR）-nucleotide binding site （NBS）-leucinerich repeat region （LRR） class of R genes and confers resistance to tobacco mosaic virus （TMV）. The N gene was isolated by insertional mutagenesis using the Activator （Ac） transposon of maize, and N-TMV interaction was one of the earliest and most studied models of plant-pathogen interaction. In this article, the research progress of N gene in structure, expression, action mechanism were reviewed from the transcription products, expression characteristics, temperature sensitivity, corresponding avirulence gene and other aspects. The efficient using and achievements of N gene in TMV resistance breeding were summarized. And the prospect of efficient using of N gene was given from three aspects, which were the mechanism research of resistant to TMV, identification and utilization of germplasm resources.
出处
《中国农学通报》
CSCD
2013年第19期89-92,共4页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"烟草病程相关蛋白PR10抗病分子机理研究"(31240013)
中国农业科学院烟草研究所所长基金"烟草病程相关蛋白PR10基因克隆及表达分析"(TR20115)
四川省烟草专卖局项目"优质特色烤烟新品种选育及推广应用"(201101004)
中国烟草总公司科技重点项目"优质抗病烤烟新品种选育及育种技术研究"(110201002002)
关键词
烟草
N基因
作用机理
遗传育种
tobacco
N gene
action mechanism
genetic breeding
