摘要
目的探讨体外分离培养人脐带源间充质干细胞（hUCMSC）的方法，并检测hUCMSC的表面标志。方法分离脐带华通胶（Wharton’sjeuy），将其剪碎后利用组织块贴壁法培养获得hUCMSC，生长至一定密度后进行传代，采用流式细胞术检测第3代hUCMSC表面标志。结果由人脐带华通胶可方便、有效地获得hUCMSC，其体外生长形态类似成纤维细胞，并可稳定增殖和传代。hUCMSC表面标志CD29、CD44、CD105高表达，而表面标志CD45、CD34、HLA-DR、HLA-G、CD80、CD86不表达。结论利用人脐带华通胶组织块培养可有效获得hUCMSC，为组织工程提供丰富的细胞来源。
Objective To investigate the methods of isolation and culture in vitro and detect the surface markers of human umbilical cord mesenchymal stem cells. Methods Human umbilical cord Wharton＂ s jelly was separated and cut up as small as possible, and then cultured with α-MEM. Human umbilical cord mesenchymal stem cells could be obtained by culturing the tissue block adhered the bottle wall. And the cells were passaged at a certain density. The surface markem of human umbilical cord mesenchymal stem cells were detected by FACS when the cells were in Generation Three. Results Human umbilical cord mesenchymal stem ceils were obtained from Wharton＂ s jelly conveniently, with fibroblast shape and stable proliferation and passage. CD29, CDu, CD105 were strongly expressed on human umbilical cord mesenehymal stem cells. But CD45, CD34, HLA-DR, HLA-G, CD80, CD86 were not expressed. Conclusion Human umbilical cord mesenchymal stem cells can be obtained effectively from the culture of the tissue block, which provides a rich source of cells for tissue engineering.
出处
《白血病．淋巴瘤》
CAS
2013年第6期354-356,共3页
Journal of Leukemia & Lymphoma
关键词
胎血
间质干细胞
细胞培养技术
抗原
表面
华通胶
Fetal blood
Mesenchymal stem cells
Cell culture techniques
Antigens, surface
Wharton＇s jelly
