摘要
目的探讨青岛地区汉族人群基质金属蛋白酶-9(MMP-9)基因rs17577多态性与脑梗死关系。方法采用多聚酶链反应-限制性内切酶片段长度多态性技术和基因序列测定技术,检测脑梗死组和对照组MMP-9基因型及等位基因频率并进行比较。结果脑梗死组MMP-9基因GA基因型频率、A等位基因频率均显著高于对照组,差异有统计学意义(χ2=8.74、7.79,P<0.01)。Logistic回归分析显示,A等位基因与脑梗死发病有关(OR=1.855,95%CI=1.206～2.854,P<0.01)。结论 MMP-9基因rs17577多态性与脑梗死可能相关,A等位基因可能是脑梗死的危险因素。
Objective To investigate the relationship between the polymorphism of matrix metalloproteinase-9 （MMP-9） gene rs17577 and cerebral infarction （CI） inHan population of Qingdao. Methods The polymorphisms of MMP-9 gene rs17577 in CI patients and normal controls were detected and compared by employing polymerase chain reaction-restriction fragment length polymorphism and gene sequencing. Results The frequency of the GA genotype and A alleles were significantly higher in CI than that in the control group （x 2 = 8.74,7.79;P 〈0.01）. Logistic regression analysis indicated that A allele was associated with the development of CI （OR=1.855,95%CI= 1.206--2.854,P〈0.01）. Conclusion The genetic polymorphism at the rs17577 site of MMP-9 gene is involved in the pathogenesis of cerebral infarction, and A allele being its risk factor.
出处
《青岛大学医学院学报》
CAS
2013年第3期202-204,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
山东省自然科学基金资助项目(ZR2009CM136)
关键词
脑梗塞
基质金属蛋白酶类
基因型
基因频率
brain infarction
matrix metalloproteinases
genotype
gene frequency
