摘要
为了研究裂蹄木层孔菌发酵产物对植物病原菌的抑菌活性。采用菌丝生长速率法测定裂蹄木层孔菌不同发酵时间的产物对7种供试病原菌的抑制作用,将裂蹄木层孔菌的发酵产物进行不同的处理,分级纯化抑菌活性多糖,比较其抑菌活性。结果表明:裂蹄木层孔菌发酵产物对7种病原菌均有抑制作用;发酵时间为20天的裂蹄木层孔菌发酵产物对杨树烂皮病的抑制率最高;裂蹄木层孔菌发酵产物的主要抑菌活性物质存在于发酵上清液醇提物中;醇沉级别为30%(浓度为50mg/mL)醇提物中的多糖对病原菌的抑制率为供试醇沉级别中最高。说明裂蹄木层孔菌含有抑制病原菌的有效成分,具备成为新型生物农药的潜力。
To discover the antifungal activity of the extracts from Phellinus linteus. We used the growth rate method measuring antifungal action to seven kinds of plant pathogen by Phellinus linteus different fermentation time fermentation product, the fermentation product was treated by different way, get the polysaccharide from the ethanol concentration, compare to antifungal activity. The results showed that antibacterial substances from Phellinus linteus had growth inhibitory effects on seven pathogenic bacteria;fermentation time was 20 d, the antibiotic compound had the highest inhibitory rate to Cytospora chrysosperma. The antibiotic compound from Phellinus linteus almost existed in supernatant of acoparia-alcohol extract;30%alcohol-precipitation concentration （concentration was 50 mg/mL） was highest in all. Phellinus linteus contained the antifungal activity component and had the potential became the new biological pesticide.
出处
《中国农学通报》
CSCD
2013年第21期78-82,共5页
Chinese Agricultural Science Bulletin
基金
黑龙江省博士后科研启动基金"药用真菌桑黄纤维素酶分子机理研究"(LBH-Q11178)
关键词
裂蹄木层孔菌
液体发酵
生物农药
抑菌活性
Phellinus linteus
liquid fermentation
biological pesticide
antimicrobial activity
