摘要
目的:评价螺旋藻多糖对人肝癌细胞株HepG2增殖的抑制作用及辐射增敏作用。方法:用MTT法检测不同时间-浓度条件下,螺旋藻多糖作用后的人肝癌细胞株HepG2细胞存活分数,计算细胞增殖抑制率;用流式细胞术检测不同时间-剂量条件下,螺旋藻多糖对X线辐射后人肝癌细胞株HepG2周期阻滞及凋亡率的影响。结果:螺旋藻多糖对人肝癌细胞株HepG2具有体外增殖抑制作用。螺旋藻多糖可将肝癌细胞阻滞于G0/G1和S期并诱导其凋亡,流式细胞仪检测有凋亡峰出现。结论:螺旋藻多糖对肝癌细胞HepG2体外增殖抑制作用表现为时间-浓度依赖性效应。一定条件下螺旋藻多糖对人肝癌细胞HepG2具有辐射增敏作用。
Objective： To evaluate the inhibition of spriulina polysacchrides to the proliferation of HepG2 human liver cancer cell line and explore its radiation sensibilization. Method： Survival fractions of HepG2 human liver cancer cell line were tested with MTT method under different times and concentrations when the cell lines were dealt with spriulina polysacchrides to calculate inhibition ratio of cellular proliferation; Effects of spriulina polysacchrides on apoptosis rate and cell cycle arrest of HepG2 human liver cancer cell line which was radiated with X-ray were detected under different times and dosages with flow cytometry. Result： Spriulina polysacchrides could inhibit the proliferation of HepG2 human liver cancer cell line in vitro. Spriulina polysacchrides could arrest liver cancer cells at G0/G1 and S phases, the detection of flow cytometry showed that there was apoptotic peak appeared. Conclusion： The effects of spriulina polysacchrides on the inhibition of HepG2 human liver cancer cell extracorporeal proliferation are time-concentration dependent relationship. Under a certain condition, spriulina polysaccharides could enhance radiation sensibilization of HepG2 human liver cancer cell.
出处
《西部中医药》
2013年第7期20-22,共3页
Western Journal of Traditional Chinese Medicine
关键词
螺旋藻多糖
肝癌细胞
增殖抑制
凋亡率
spriulina polysacchrides
hepatoma carcinoma cells
proliferation inhibition
apoptosis rate
