摘要
以美国内华达州大盆地温泉采集样品为材料,富集获得纤维素及半纤维素高效稳定降解厌氧菌群SVY42,以巨菌草、甘蔗渣、废菇筒、羧甲基纤维素钠、滤纸、木聚糖为碳源,分析菌群SVY42产内切葡聚糖酶(CMC酶)、β-葡萄糖苷酶和木聚糖酶的情况。在此基础上,以木聚糖为底物筛选高产木聚糖酶的菌株。菌群SVY42在以巨菌草作为碳源时的β-葡萄糖苷酶活最高为0.23 U/mL,以木聚糖作为碳源时CMC酶活和木聚糖酶活均为最高,分别为0.31 U/mL和0.35 U/mL。从菌群SVY42中筛选得到1株高产木聚糖酶厌氧菌株SVY42-1,该菌在最适温度41℃和pH 8.0条件下,其木聚糖酶活力为0.26 U/mL,对其进行16S rDNA序列系统进化分析,SVY42-1与已知菌株的最高同源性仅为93.81%,初步鉴定属于新属。
A highly effective and stable cellulose and hemicellulose degradable anaerobic bacterial population（ABP） SVY42 from the samples collected from Great Basin Hot Spring in Nevada,USA as material was enriched with and obtained.Their production conditions of CMCase,β-galactosidase and xylanase by ABP SVY42 were studied with the giant Juncao,bagasse,waste mushroom culturing cylinder,sodium carboxymethyl cellulose（CMC）,filter paper and xylan as carbon sources.Based on these,a xylanase high-producing strain was screened using xylan as substrate.The highest β-galactosidase activity was 0.23 U/mL using the giant Juncao as substrate.The highest CMCase and xylanase activities were 0.31 U/mL and 0.35 U/mL using xylan as substrate respectively.A xylanase high-producing thermophilic strain SVY42-1 from ABP SVY42 was screened from ABP SVY42.The xylanase activity reached 0.26 U/mL under the optimal temperature（41 ℃） and pH（8.0）.Strain SVY42-1 was identified by 16S rDNA sequencing analysis and only 93.8% similar to the highest homology of the known strains,and identified initially belong to a new genus.
出处
《微生物学杂志》
CAS
CSCD
2013年第3期49-53,共5页
Journal of Microbiology
基金
国家自然科学基金(31071639)
中国博士后科学基金(2013M530303)
福建省校企合作项目(2010N5005)
福建省教育厅科技项目(JB12055)
福建农林大学青年教师科研基金重点项目(2012XJJ03)
国家海洋局海洋生物遗传重点实验室开放研究基金(HY201301)
关键词
厌氧菌群
产酶分析
木聚糖酶
分离鉴定
anaerobic bacterial population（ABP）
enzyme production analysis
xylanase
isolation and identification
