摘要
目的:观察消癌舒(XAS)的抗炎镇痛作用并分析其作用机制。方法:昆明种小鼠随机分为模型组,阿司匹林组(0.2 g.kg-1),XAS低、中、高剂量组(0.375,0.75,1.5 g.kg-1),连续灌胃给药7 d。采用二甲苯致小鼠耳廓肿胀及角叉菜胶致小鼠足肿胀实验评价XAS的抗炎作用;采用冰醋酸致痛法及热板致痛法评价XAS的镇痛作用;采用Real time RT-PCR法测定炎性组织中肿瘤坏死因子α(TNF-α)及白介素1β(IL-1β)mRNA的表达。结果:XAS能明显抑制小鼠耳廓肿胀及足肿胀程度(P<0.05);对抗热板及醋酸刺激所致的小鼠疼痛反应(P<0.05);降低炎症组织中TNF-α及IL-1βmRNA的表达(P<0.05)。结论:XAS具有显著的抗炎、镇痛作用,其作用机制与抑制炎性组织中TNF-α,IL-1βmRNA的表达有关。
Objective： To observe the anti-inflammatory and analgesic effects of Xiaoaishu（XAS） and explore it＇s mechanism.Method： The KM mice were randomly divided into model group,aspirin group（0.2 g.kg-1） and XAS groups（0.375,0.75,1.5 g.kg-1） ig,for 7 d.Carrageenan-induced paw swelling and dimethylbenzene-induced pinna swelling in mice were used to evaluate the anti-inflammatory effects.The analgesic effects of XAS were tested by counting writhing frequency induced by acetic acid and measuring the latent period of licking hind foot with the hot plate method in mice.The levels of tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β） mRNA was determined by real time RT-PCR.Result： XAS significantly suppressed the dimethylbenzene-induced mice auricle swelling and Carrageenan-induced mice paw edema（P 0.05）,XAS significantly reduced the acetic acid-induced writhing frequency and pain threshold in mice（P 0.05）,real-time RT-PCR showed that XAS could decrease the level of TNF-α and IL-1β mRNA of inflammatory tissue in mice（P 0.05）.Conclusion： XAS have evident anti-inflammatory and analgesic effects,the mechanism may be related with the inhibition of the expression of TNF-α and IL-1β mRNA in the carrageenen-induced mice paw.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第14期199-202,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技厅中药现代化专项(黔科合中药字20115008号)
贵州省教育厅自然科学类科研项目(黔教科2011056号)
贵州省科技厅自然科学基金(黔科合J字20092147号)
贵州省中医药管理局项目(QZYY2010-59)
关键词
XAS
抗炎
镇痛
细胞因子
XAS
anti-inflammatory
analgesia
cytokines
