摘要
目的:揭示地黄饮子抗脑缺血再灌注损伤的作用机制。方法:将SD大鼠60只随机分为6组,即假手术组、模型组、阳性药尼莫地平组(1 mg.kg-1)和地黄饮子高、中、低(32,16,8 g.kg-1)剂量组,每组10只,用药7 d后造模。通过双侧颈总动脉结扎法制作SD大鼠脑缺血再灌注模型,观察地黄饮子对大鼠脑缺血再灌注120 min后血清、脑组织超氧化物歧化酶(S0D)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活力及丙二醛(MDA)含量的影响。结果:模型组大鼠血清、脑组织SOD,CAT和GSH-Px活性明显降低,MDA含量明显增加,地黄饮子能显著抑制模型大鼠血清、脑组织中SOD,CAT和GSH-Px活力的降低及MDA含量的升高。结论:地黄饮子可通过减轻自由基损伤、提高脑组织抗氧化能力达到抗脑缺血再灌注损伤的作用。
Objective： To explore the mechanism of Dihuang Yinzi in ameliorating the damage induced by cerebral ischemia-reperfusion.Method： Sixty SD rats were selected and randomly divided into six groups： sham group,model group,Dihuang Yinzi high,medium,low dose group（32,16,8 g.kg-1） and nimodipine positive drug group（1 mg.kg-1）,each group include 10 rats.The cerebral ischemia-reperfusion model was established after 7 days drug administration.The activities of superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-Px） and the content of malonaldehyde（MDA） in the serum and brain of rats were observed at 120 min after the cerebral ischemia-reperfusion.Result： The activitis of SOD,CAT and GSH-Px were lower and the contents of MDA were higher in serum and brain of mode1 rats than normal rats.Dihuang Yinzi could increase the activitis of SOD,CAT and GSH-Px and decrease the content of MDA.Conclusion： Dihuang Yinzi can ameliorate the oxidative damage induced by cerebral ischemia-reperfusion.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第14期247-250,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山东省高等学校科技计划项目(J12LM54)
关键词
地黄饮子
脑缺血
再灌注损伤
抗氧化
Dihuang Yinzi
cerebral ischemia
reperfusion injury
antioxidant
