摘要
目的:研究血三七水煎剂的抗血栓作用及其作用机制。方法:观察小鼠给予不同剂量的(剂量分别为生药0.4,0.2,0.1 g.kg-1)血三七水煎剂,7 d后对其凝血时间的影响;采用胶原蛋白-肾上腺素混合溶液诱导小鼠形成血栓,分别ig给予0.5 g.kg-1血栓心脉宁片(阳性对照组)、不同剂量的血三七水煎剂(剂量分别0.6,0.4,0.2 g.kg-1),模型组给予等体积生理盐水,每天1次,连续7 d,观察给药后对小鼠偏瘫和死亡率的影响;制备大鼠血瘀证模型,ig给予血三七水煎剂,连续15 d后取血,测定血浆中血栓素B2(TXB2)、6-酮前列腺素F1α(6-keto-PGF1α)含量、血浆纤溶酶原激活物(t-PA)、纤溶酶原激活抑制物(PAI)含量及对二磷酸腺苷(ADP)、花生四烯酸(AA)、胶原(COL)诱导的血小板聚集的影响。结果:血三七水煎剂高、中、低剂量组的小鼠凝血时间分别为(164±23),(143±20),(112±21)s,均能明显延长小鼠凝血时间;血三七水煎剂高、中、低剂量组血栓模型小鼠死亡数分别1,2,2只,偏瘫数分别为2,2,3只,与模型组比较存在显著性差异;血三七水煎剂可促使血瘀证模型大鼠血浆TXB2,6-Keto-PGF1α,t-PA含量均减少,PAI含量升高,抑制血小板聚集。结论:血三七水煎剂具有较强的抗血栓作用,具体作用机制可能与影响血小板功能和聚集相关。
Objective： To observe the antithrombotic effects of Polygonum amplexicaule decoction and the action of mechanism.Method： After orally given different doses of P.amplexicaule decoction（crude drug dose： 0.4,0.2,0.1 g.kg-1） for 7 days,the clotting time of each group mice was determined;thrombus induced by collagen-epinephrine were established,positive control group was orally given 0.5 g.kg-1 Xueshuan Xinmaining tablets,experimental drug group were orally given different doses of P.amplexicaule decoction（crude drug dose： 0.6,0.4,0.2 g.kg-1）,model group was given equal volume normal saline once a day for 7 d,then thrombosiscaused hemiplegia and death of each group were determined.Experimental models of artery thrombosis was prepared,they were given P.amplexicaule decoction by intragastric administration for 15 days.Then the blood was taken,the content of plasma thromboxane B2（TXB2）,6-keto-prostaglandin F1α（6-Keto-PGF1α）,tissue-type plasminogen activator（t-PA） and plasminogen activator inhibitor（PAI） were determined.Effect of P.amplexicaule decoction on antiplatelet aggregation induced by ADP,AA and COL were also investigated.Result： The hemostasis time of mice after given high,middle and low doses of P.amplexicaule decoction was（164 ± 23）,（143 ±20）,（112 ±21） s,P.amplexicaule decoction could prolong the clotting time of mice;mortality data of thrombotic mice after given high,middle and low doses of P.amplexicaule decoction was 1,2,2,and hemiplegia data of that was 2,2,3,compared with the model group there was significant difference;the content of TXB2,6-Keto-PGF1α,t-PA of blood-stasis model rats could reduce after given P.amplexicaule decoction,content of PAI increased,and inhibite platelet aggregations.Conclusion： P.amplexicaule decoction has obvious antithrombotic effects,which was probably related to platelet functions and aggregations.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第14期256-259,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
血三七
水煎剂
抗血栓
作用机制
Polygonum amplexicaule
decoction
antithrombotic
mechanism
