摘要
用 0～ 70 0mmol/LNaCl和 0～ 1 50mmol/LNa2 CO3对羊草 (Aneu rolepidiumchinense (Trin)Kitag .)苗进行胁迫处理 ,测定其N及几种有机渗透调节物的积累情况 .结果表明 :羊草对两种盐胁迫的反应明显不同 .对于NaCl,可耐受的最大强度为 60 0mmol/L ;对于Na2 CO3,可耐受的最大强度为 1 2 5mmol/L .相同Na+胁强下 ,Na2 CO3胁迫使羊草苗体内含N量下降的幅度明显大于NaCl胁迫 .NaCl胁迫下 ,脯氨酸及可溶性糖含量随Na+胁强增大呈直线式平缓上升 ,在Na2 CO3胁迫下则呈明显的曲线变化 ;在胁强较低时 ,脯氨酸可溶性糖含量无明显变化 ,但当胁强超过 87 5mmol/L时 ,二者均转而急剧上升 ,其中尤以脯氨酸含量的变化最明显 .NaCl胁迫时 ,羊草中有机酸及柠檬酸含量无明显变化 ,而Na2 CO3胁迫则使二者含量明显上升 ,并证明积累的有机酸以柠檬酸为主 .从实验结果看来 ,中性盐 (NaCl)与碱性盐 (Na2 CO3)对羊草的胁迫机制以及羊草对其生理适应机制存在明显差异 .
Using Aneurolepidium chinense as the research object, stressed by NaCl(0～700 mmol/L) and Na 2CO 3(0～150 mmol/L). The experiment was carried out about determining the content of nitrogen and organic osmoticajustment matters. Results show that: A.chinense reacted differently to saline-alkaline stress. The density which A.chinense was 125 mmol/L under Na 2CO 3 stress. At the same of [Na +],the nitrogen content of A.chinense decreased more clearly under Na 2CO 3 stress. With [Na +] increasing, the content of proline and soluble sugar increased linely under NaCl stress, the content of proline and soluble sugar increased curvely under Na 2CO 3 stress. That is to say, at the low density, the content of proline and soluble sugar changed hardly. But after exceeding the density of 87.5 mmol/L, both of them increased quickly. The content of organic acid and citric acid didn't increase dearly under NaCl stress, and both of them increased obviously under Na 2CO 3 stress. At the same time, we proved that one of the most important organic acids was citric acid. Experiments show that: there was obviouse difference in the mechanism of saline-alkaline stress. it was different for A.chinense to adapt saline-alkaline stress in physiological mechanism.
出处
《东北师大学报（自然科学版）》
CAS
CSCD
2000年第3期47-52,共6页
Journal of Northeast Normal University(Natural Science Edition)
