摘要
为探讨不同基因型茶菊(tea Chrysanthemum)在盐胁迫下的生理响应并对其进行耐盐性评价,以4个不同基因型茶菊为材料,采用营养液浇灌法,研究了不同浓度NaCl(0、40、80、120、160、200mmol·L-1)胁迫下茶菊生理生化和光合生理响应特性。结果表明:随着NaCl胁迫程度加大,不同基因型茶菊叶片细胞膜透性(Cond)、丙二醛(MDA)含量、叶片脯氨酸(Pro)含量和可溶性糖(SS)含量增加;超氧化物歧化酶(SOD)含量呈先升后降趋势;‘乳荷’、‘黄滁龙’叶绿素(Chl)含量持续下降,‘繁白露’和‘玉人面’叶绿素含量呈先升后降的趋势;净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Gs)随NaCl胁迫浓度提高而极显著降低,气孔限制值呈先升后降的趋势。采用隶属函数法对茶菊进行耐盐性评价,不同基因型茶菊耐盐性由强到弱依次为‘乳荷’>‘玉人面’>‘繁白露’>‘黄滁龙’。其中,耐盐性品种‘玉人面’、‘乳荷’在NaCl胁迫下,Chl含量、Pn、Tr和Gs下降幅度小,MDA含量和气孔限制值增幅较小。
Aims Our objective was to investigate the physiological response of tea Chrysanthemum cultivars to salt stress in order to select salt-tolerant cultivars for use in saline soil. Methods Stress physiological, biochemical and photosynthetic physiological parameters were measured for four tea Chrysanthemum cultivars, which were irrigated with nutrient solution adding different concentrations of NaC1 （40, 80, 120, 160 and 200 mmol.L-1） as treatment and without addition of NaC1 as control. Important findings Following the increase with the degree of NaCl stress, data of relative membrane permeabil- ity （Cond）, malonic aldehyde （MDA）, proline （Pro） and soluble sugar （SS） content were increased in leaves of different genotypes of tea Chrysanthemum, while superoxide dismutase （SOD） activity was initially increased and then decreased. Leaf chlorophyll （Chl） content of the ＇Fanbailu＇ and ＇Yurenmian＇ cultivars were initially in- creased then decreased, but continued to decline in the ＇Ruhe＇ and ＇Huangchulong＇ cultivars. Net photosynthetic rate （Pn）, transpiration rate （Tr） and stomatal conductance （Gs） were significantly reduced, but the stomatal limita- tion value was first increased and then decreased following the increase of salt concentrations. Values of subordi- nate function were used to evaluate the salt tolerance among different tea Chrysanthemum cultivars. The salinity tolerance for those cultivars was ＇Ruhe＇ 〉 ＇Yurenmian＇ 〉 ＇Fanbailu＇ 〉 ＇Huangchulong＇. Under NaC1 stress, pa- rameters for Chl, Pn, Tr and Gs were decreased less in ＇Yurenmian＇ and ＇Ruhe＇ than in the other two cultivars while parameters for MDA content and stomatal limitation value were increased less in ＇Yurenmian＇ and ＇Ruhe＇ than in the other two cultivars.
出处
《植物生态学报》
CAS
CSCD
北大核心
2013年第7期656-664,共9页
Chinese Journal of Plant Ecology
基金
国家自然科学基金(30972021)
山西省攻关项目(20120311016-2)
高等学校优秀青年学术带头人支持计划(201002)
关键词
NACL胁迫
光合生理
生理响应
耐盐性
茶菊
NaCl stress, photosynthetic physiology, physiological response, salt tolerance, tea Chrysanthemum
