摘要
目的:建立复方两面针含片中苦参碱和没食子酸的HPLC含量测定方法。方法:色谱柱为ZORBAX Eclipse XDB C18(250 mm×4.6 mm,5μm)柱;流动相为乙腈-甲醇-磷酸盐缓冲液(pH 6.8)-三乙胺(16∶16∶68∶0.1)和乙腈-0.1%磷酸0.1%三乙胺水溶液(3∶97),流速1.0 mL.min-1;柱温30℃;检测波长220 nm。结果:苦参碱在0.10～2.00μg范围内线性关系良好(r=0.99960,n=6);平均回收率为99.4%(n=9)。没食子酸在0.011～1.75μg范围内线性关系良好(r=0.99998,n=6);平均回收率为99.1%(n=9)。结论:建立的方法适用于复方两面针含片的质量控制。
Objective： To establish an analytical method for determination of matrine and gallic acid in Compound Liangmianzhen tablets by HPLC.Methods： The analysis was achieved on a ZORBAX Eclipse XDB C18 column（250 mm ×4.6 mm,5 μm） using a mobile phase of acetonitrile-methanol-phosphate buffer（pH 6.8）-triethylamine（16∶ 16∶ 68∶ 0.1） and a mobile phase of acetonitrile-0.1% phosphoric acid and 0.1% triethylamine water solution（3∶ 97） as the mobile phase;the flow rate was 1.0 mL.min-1;the detecting wavelength was 220 nm and the column temperature was 30 ℃.Results： The calibration curve of matrine was linear within the range of 0.10-2.00 μg（r =0.9996,n =6）,theaverage recovery was 99.4%（n =9）.The calibration curve of gallic acid was linear within the range of 0.011-1.75 μg（r = 0.9999,n = 6）,the average recovery was 99.1%（n = 9）.Conclusion： The method can be used to control the quality of the Compound Liangmianzhen tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第7期1259-1262,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
复方两面针含片
西青果
山豆根
没食子酸
苦参碱
高效液相色谱法
Compound Liangmianzhen tablets
Fructus terminaliae immaturus
Sophora tonkinensis
Gallic acid
matrine
HPLC
