摘要
目的 探讨低浓度活性氧对多巴胺能神经元增殖的影响.方法 实验组分别用低浓度（10、20和30 μmol/L）过氧化氢（H2O2）干预人神经母细胞瘤细胞SH-SY5Y 24 h,对照组加入等体积培养基.应用MTT法检测各组细胞活性;比色法测定各组细胞乳酸脱氢酶（LDH）释放率.结果 低浓度H2O2干预SH-SY5Y细胞24 h后,10 μmol/L H2O2组、20 μmol/L H2O2组和30 μmol/L H2O2组吸光度A570值分别为1.011 2±0.015 3、1.119 1±0.035 3和1.027 1±0.035 2,与对照组（0.959 0±0.034 7）比较,差异均有统计学意义（均P＜0.05）;10、20和30 μmol/L H2O2组之间两两比较,差异均有统计学意义（F=18.727,P＜0.05）,其中20 μmol/L H2O2组促进SH-SY5Y细胞增殖效果最为明显.10、20和30 μmol/L H2O2组LDH释放率分别为（26.00±1.09）％、（24.62±0.77）％、（25.88±0.95）％,与对照组LDH释放率（27.86±1.31）％相比,差异均具有统计学意义（均P＜0.05）;10、20和30 μmol/L H2O2组之间两两比较,差异无统计学意义（F=3.254,P=0.074）.结论 低浓度活性氧可能对多巴胺能神经元的增殖有促进作用.
Objective To investigate the effects of low concentrations of reactive oxygen species on dopaminergic neuron proliferation. Methods The human neuroblastoma cells SH- SY5Y were incubated with low concentrations （ 10, 20 and 30 μmol/L） of H202 for 24 h in the study group and an aliquot of culture media in the blank control group, respectively. The viability of SH- SY5Y cells was assayed by the MTT method, and the release of LDH was determined by the colorimetric assay. Results Following treatment with low concentrations of H202 for 24 h, the SH-SY5Y cells yielded a significantly higher absorption （A570） 1.011 2±0.015 3, 1.119 1±0.035 3 and 1.027 1±0.035 2 corresponding to 10, 20 and 30 txmol/L H202 when compared with control group 0.959 0±0.034 7 （all P〈0.05） respectively. The between-group difference in study groups was statistically significant （F=18.727, P〈0.05）. The 20 p.mol/L group showed the most significant effect on cell proliferation. Treatment with 10, 20 and 30 μmol/L H202 was characterized by considerably higher release of LDH （26.00± 1.09）%, （24.62±0.77）% and （25.88±0.95）% compared with control group （27.86± 1.31 ）% （all P〈0.05 ）. However, there was no between-group difference in the release of LDH among the study groups （F=3.254, P=0.074）. Conclusion Low concentrations of reactive oxygen species may promote dopaminergic neuron proliferation.
出处
《中华生物医学工程杂志》
CAS
2013年第2期117-119,共3页
Chinese Journal of Biomedical Engineering
基金
广东省科技计划项目（20128031800312）
