摘要
目的探讨缺氧诱导因子(HIF)-1α和血管内皮生长因子(VEGF)在稽留流产患者血清和绒毛中的表达及二者的相关性。方法采用酶联免疫吸附试验和免疫组化SP法分别测定55例稽留流产患者(流产组)和45例正常早孕孕妇(对照组)血清和绒毛中HIF-1α和VEGF的表达水平。并将稽留流产组按有无明确原因分为有原因组和无原因组,同时根据胚胎死亡后稽留宫内时间分为<2周组、2～4周组和>4周组。结果流产组患者血清中HIF-1α和VEGF水平分别为(316.2±117.4)ng/L和(33.8±5.5)ng/L,对照组分别为(417.1±120.0)ng/L和(50.7±52.7)ng/L,两组比较,差异有统计学意义(P<0.05);流产组患者绒毛中HIF-1α和VEGF的表达量分别为3.0±2.1和3.6±1.9,对照组分别为6.2±3.4和6.8±3.6,两组比较,差异有统计学意义(P<0.05)。Pearson相关分析显示,流产组和对照组血清中HIF-1α和VEGF的表达成呈正相关(r=0.565,P<0.05;r=0.549,P<0.05),两组绒毛中HIF-1α和VEGF的表达呈正相关(r=0.352,P<0.05;r=0.457,P<0.05)。但稽留宫内时间<2周组、2～4周组和>4周组三组血清和绒毛中HIF-1α和VEGF的表达比较,差异均无统计学意义(P>0.05)。结论 HIF-1α和VEGF在血清和绒毛中的低表达可能是导致稽留流产的重要原因之一。
Objective To investigate the expression and correlation of hy-poxia-inducible factor （HIF） -1α and vas- cular endothelial growth factor （VEGF） in serum and villus in missed abortion. Methods Expression of HIF-1α and VEGF in serum and villus in 55 patients of missed abortion and 45 patients of normal pregnancy were determined by using enzyme - linked immunosorbent assay and irnmunohistochemistry. Depending on whether there was a clear reason, missed abortion group was divided into two groups. According to different time, missed abortion group was further divide into 〈2 weeks group, 2-4 weeks group and 〉4 weeks group. Results Expression of HIF-1α and VEGF in serum and villus in missed abortion patients were significantly lower than that in control group （P〈0. 05）. Pearson correlation analysis showed that expression of HIF-1α and VEGF in serum in the two groups had a positive correlation （r =0. 565, P〈0. 05; r=0. 549, P〈0.05） while the expression of HIF-1α and VEGF in the villus had a positive correlation （r=0. 352, P〈0. 05; r= 0. 457, P〈0. 05）, but there were no significant difference of HIF-1α and VEGF levels both in serum and in villus among the three different time groups （P〉0. 05）. Conclusion The low expression of HIF-1α and VEGF in serum and villus may play an important role in missed abortion.
出处
《中国妇产科临床杂志》
2013年第4期315-319,共5页
Chinese Journal of Clinical Obstetrics and Gynecology
关键词
稽留流产
血清
绒毛
缺氧诱导因子1Α
血管内皮生长因子
missed abortion
serum
villus
hypoxia inducible factor 1α
vascular endothelial growth factor
