摘要
【目的】分析贵州省各生态区烟蚜(Myzus persicae)种群间是否存在遗传差异及其差异程度,探明贵州烟蚜种群异质性及种群分化情况,揭示变异发生规律及其机理,为指导虫情监测以及综合治理提供理论依据。【方法】用SSR分子标记技术对贵州省25个主要烟区的烟蚜种群进行遗传多样性分析。【结果】25个地理种群中大部分的种群呈中度分化,个别种群呈高度分化;种群遗传分化及与地理距离、海拔之间的关系分析表明,种群间的遗传距离与地理距离无显著相关性,遗传一致度与海拔差距无相关性。烟蚜种群的系统发育分析表明,25个地理种群大致分为3部分。【结论】种群的遗传分化不符合地理隔离模式,贵州省烟蚜各种群的种群分化复杂,其原因可能与贵州省特殊的地形和气候有关。
[Objective] The objective of this study is to analyze whether there are genetic differences and the degree of difference of Myzus persicae in typical ecological areas of Guizhou, and to provide an important theoretical basis for clearing the population heterogeneity and population differentiation, discovering and revealing the variation rule and mechanism, guiding the pest's forecast as well as integrated management. [Method] SSR molecular marker technology was used for genetic diversity analysis of tobacco aphid populations of 25 major tobacco-growing areas in Guizhou Province. [Result] Most of the populations were moderately differentiated, and minority populations were highly differentiated. Through analysis, the relationship of population genetic differentiation, geographic distance and the altitude were that the genetic distance and geographic distance had no significant correlation, genetic similarity and the elevation had no significant correlation either. The cluster analysis showed that the 25 populations could be divided into 3 parts. [Conclusion] The population genetic differentiation is not in conformity with the geographical isolation mode. Genetic differentiation of the 25 populations in Guizhou is complex. The special terrain and climate may be the reason.
出处
《中国农业科学》
CAS
CSCD
北大核心
2013年第13期2685-2694,共10页
Scientia Agricultura Sinica
基金
中国烟草总公司贵州省公司2010年度科学研究与技术开发项目计划(201022)
关键词
贵州
烟蚜
微卫星标记
遗传多样性
遗传分化
Guizhou Province
Myzus persicae (Sulzer)
microsatellite marker
genetic diversity
genetic differentiation