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白芍总苷对腹膜纤维化大鼠转化生长因子-β1的影响 被引量:4

Effect of Total Glucosides of Paeony on Transforming Growth Factor Beta 1 in Peritoneal Fibrosis Rats
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摘要 【目的】探讨白芍总苷(the total glucosides of paeony,TGP)对腹膜纤维化大鼠转化生长因子-β1(TGF-β1)的影响。【方法】选用健康SD雄性大鼠32只,随机分为4组,每组8只,分别为空白组、模型组、白芍总苷高剂量组、白芍总苷低剂量组。采用腹腔注射42.5 g/L含糖透析液+脂多糖法复制腹膜纤维化模型。白芍总苷高、低剂量组造模同时给予白芍总苷灌胃(剂量分别为200、100 mg.kg-1.d-1);实验第30天结束后留取腹膜组织和腹透液,采用一步法Real-time PCR测定腹膜组织TGF-β1 mRNA的表达,采用酶联免疫吸附(ELISA)法测定腹透液中TGF-β1的水平。【结果】模型组腹膜TGF-β1mRNA的表达及腹膜透析液TGF-β1的水平显著高于空白组(P<0.001),白芍总苷高、低剂量组2指标水平较模型组均显著降低(P<0.05)。【结论】白芍总苷可以上调TGF-β1的表达,对腹膜纤维化具有一定防治作用。 Objective To explore the effect of the total glucosides of paeony(TGP) on transforming growth factor beta 1(TGF-β1) in peritoneal fibrosis rats.Methods Thirty-two healthy SD male rats were equally randomized into 4 groups,and they were blank control group,model group,high-dose and low-dose TGP groups.Rat model with peritoneal fibrosis was induced by intraperitoneal injection of 42.5 g/L high-glucose dialysate combined with lipopolysaccharide(LPS).The rats in TGP groups were given gastric gavage of 100,200 mg·kg^-1·d^-1 TGP respectively during the modeling.On experiment day 30,all rats were sacrificed,and peritoneal tissue and peritoneal dialysis fluid were sampled.One-step real-time polymerase chain reaction(PCR)was used to examine TGF-β1 mRNA in the visceral peritoneum,and TGF-β1 level in the peritoneal dialysis fluid was detected by enzyme-linked immunospecific assay(ELISA)method.Results TGF-β1 mRNA expression and TGF-β1 level were increased in the model group than those in the blank control group(P〈0.001),and were decreased in high-dose and low-dose TGP groups(P〈0.05 compared with the model group).Conclusion TGP can decrease the expression of TGF-β1 in the peritoneum,and show certain preventive and therapeutic effect on peritoneal fibrosis.
出处 《广州中医药大学学报》 CAS 北大核心 2013年第4期511-514,607,共5页 Journal of Guangzhou University of Traditional Chinese Medicine
基金 广东省自然科学基金资助项目(编号:S2011010005292)
关键词 腹膜纤维化 中药疗法 白芍总苷 药理学 TGF-Β1 基因表达调控 疾病模型 动物 大鼠 Peritoneal fibrosis/TCD therapy Total glucosides of paeony/pharmacology TGF-β1 Gene expression regulation Disease models animal Rats
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参考文献12

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