摘要
目的观察2-脱氧-D-葡萄糖(2-DG)标记的纳米粒(γ-Fe2O3@DMSA-DG NPs)对高代谢肺腺癌A549细胞的靶向吸收效果,探讨其作为一种新型的磁共振靶向对比剂的可行性。方法制备γ-Fe2O3@DMSA-DG NPs并对其理化性质进行研究,采用普鲁士蓝染色定性及比色法定量比较高代谢A549细胞分别与γ-Fe2O3@DMSA-DG和γ-Fe2O3@DMSA NPs孵育在不同时间(10 min,30 min,1 h,2 h)的吸收差异,及在游离D-葡萄糖的竞争下观察A549细胞对γ-Fe2O3@DMSA-DG的吸收情况,MRI常规序列和T2-mapping序列观察T2WI信号强度和色阶变化。结果普鲁士蓝染色法显示A549细胞γ-Fe2O3@DMSA-DG组蓝染颗粒多于γ-Fe2O3@DMSA组。比色法检测也显示在不同的时间点γ-Fe2O3@DMSA-DG组细胞内铁含量均高于γ-Fe2O3@DMSA组,两者间差异有统计学意义(P<0.05),MRI检测显示在各时间点A549细胞γ-Fe2O3@DMSA-DG组信号强度低于γ-Fe2O3@DMSA NPs组,γ-Fe2O3@DMSA-DG组T2-mapping值降低明显(10 min、30 min、1 h、2 h时,T2-mapping值分别降低7.43%,46.40%,62.66%,81.07%,而γ-Fe2O3@DMSA组T2-mapping值降低相对较小(10 min、30 min、1 h、2 h时,T2-map-ping值分别降低0.79%,12.49%,43.54%,77.71%。在游离D-葡萄糖的竞争下,A549细胞γ-Fe2O3@DMSA-DG组蓝染颗粒明显减少,细胞内铁含量与无D-葡萄糖的竞争下细胞铁含量差异有统计学意义,MRI信号强度降低和色阶变化不大。结论携带2-DG的磁共振对比剂(γ-Fe2O3@DMSA-DG NPs)对高代谢肺腺癌A549细胞有显著的靶向吸收效果,可作为一种新型的磁共振肿瘤靶向对比剂。
Objective To observe the targeting uptake of 2 deoxy D glucose coated nanoparticles to lung carcinoma A549 cells with highly metabolism level, and to investigate the feasibility of serving as a new type MRI targeted contrast a- gent. Methods γ-Fe2O3@ DMSA DG was prepared and the physical and chemical properties were detected. The uptake difference ofγ-Fe2O3@ DMSA DG and γ-Fe2O3@ DMSA NPs were visualized and measured quantitatively for lung carcino- ma cells （A549） with highly metabolism level for different time interval （10 min, 30 min, 1 h ,2 h）. The uptake level of γ-Fe2O3@ DMSADG NPs was also observed by competition of D glucose. MRI was performed to visualize the signal intensity changes via T2WI sequence and T2 mapping sequences. Results Prussian blue staining showed higher intracelluar blue granule amount of γ-Fe2O3@ DMSA DG group than that of γ-Fe2O3@ DMSA NPs group for lung carcinoma cells. As re- vealed by UV colorimetric assay, highly metabolic A549 cells also showed that the cellar iron amount of γ-Fe2O3@ DMSA DG group was significantly higher than that of γ-Fe2O3@ DMSA group, the difference was significant statistically between two groups （P 〈0.05）. MRI showed that the decrease of signal intensity of γ-Fe2O3@ DMSA DG group was lower than that of γ-Fe2O3@ DMSA group, the T2 mapping value of γ-Fe2O3@ DMSA DG group was decreased significantly （ the T2 mapping value decreased by 7.43% ,46.40% ,62.66% ,81.07% for 10 min, 30 min,1 h,2 h, respectively）, in contrast, the signal intensity did not show obvious decrease for γ-Fe2O3@ DMSA group （the T2 mapping value decreased by 0.79% , 12.49% ,43.54% ,77.71% for 10 min,30 min,1 h,2 h, respectively）. However, the same cells incubated with the sameamount of 3,-Fez 03 @ DMSA DG NPs resulted in dramatically little intracelluar blue granule amount, little signal intensity distinction in the presence of D glucose relative to absent of D glucose. Conclusion 2 deoxy D glucose coated nanoparti- cles have significant targeting to highly metabolism lung carcinoma A549 cells, and it is feasibility to develop as a specific MRI targeted contrast agent for the early diagnosis of lung carcinoma in the future.
出处
《临床放射学杂志》
CSCD
北大核心
2013年第7期1044-1049,共6页
Journal of Clinical Radiology
