摘要
目的研究脑神康胶囊对糖尿病大鼠记忆能力及海马乏氧诱导因子(HIF-1α)表达的影响。方法将36只大鼠分为正常对照组、糖尿病组、中药治疗组,中药治疗组用脑神康胶囊水溶液灌胃,其余两组用等量生理盐水灌胃,每日1次,持续12周。在0、4、8、12周测血糖及进行Morris水迷宫实验,使用免疫组化及RT-PCR技术检测海马HIF-1α的表达。结果中药治疗组与糖尿病组大鼠HIF-1α表达量较正常对照组增高,中药治疗组第8、12周较糖尿病组HIF-1α表达量减少(P<0.01或P<0.05)。水迷宫潜伏期自第4周始中药治疗组较糖尿病组缩短(P<0.01或P<0.05),较正常对照组延长。结论脑神康胶囊能下调HIF-1α表达,改善记忆能力下降症状,减轻糖尿病脑功能损害。
Objective To study the effect of the Naoshenkang capsule on memory and expression of hypoxia inducible factor-lalpha （HIF-1α） in hippocampus tissue of diabetic rats. Methods Thirty-six rats were divided into three groups： the control group, the diabetic group, and the Naoshenkang group. Naoshenkang capsule was administered to the Naoshenkang group once daily, and an equal volume of normal saline was given to the control and the diabetic groups. All operations continued for 12 weeks. Morris water maze was operated every four weeks. Meanwhile, the ex- pression of HIF-1α in hippocampus tissue was detected by immunohistochemistry and RT-PCR. Results The expres- sion of HIF-1α increased both in the diabetic and the Naoshenkang groups. Compared with the diabetic group, the Naoshenkang group had an obvious decrease in the expression of HIF-1α since the eighth week （ P 〈 0.01 or P 〈 0.05 ）, and it also had a reduction in the latency period of Morris water maze since the fourth week （ P 〈 0.01 or P 〈 0.05 ）. Conclusion The Naoshenkang capsule can effectively down-regulate the expression of HIF-1α and improve the memory ability, and finally ameliorate the brain damage caused by diabetes.
出处
《山东大学学报（医学版）》
CAS
北大核心
2013年第7期20-24,共5页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金(81173250)
山东省科技厅自然基金(ZR2010HM077)
关键词
脑神康胶囊
糖尿病
乏氧诱导因子
海马
水迷宫
Naoshenkang capsule
Diabetes mellitus
Hypoxia inducible factor-l alpha
Hippocampus
Morris water maze
