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人乳头瘤病毒E6/E7 mRNA检测联合宫颈活检在宫颈病变中的应用价值 被引量:4

Application value of human papilloma virus E6/E7 mRNA detection combined with cervical biopsy in diagnosis of cervical intraepithelial neoplasia
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摘要 目的探讨经过新柏超薄液基细胞学检测(TCT)的标本检测高危人乳头瘤病毒(HPV)E6/E7 mRNA联合宫颈活检在宫颈病变筛查中的可行性及应用价值。方法采用杂交信号放大的核酸检测技术,对100例TCT示不同程度宫颈上皮瘤样病变和宫颈癌的标本进行高危HPV E6/E7 mRNA检测,并对HPV E6/E7 mRNA阳性者和阴性者同时行宫颈活检,同时就HPV E6/E7 mRNA表达在不同细胞学级别中的分布情况行统计学分析。结果不同细胞诊断级别E6/E7转录数有统计学差异(P<0.05),随着宫颈病变程度增加,HPV E6/E7 mRNA的转录数相应增加。HPV E6/E7mRNA的转录数越高,宫颈活检阳性率越高。结论高危HPV E6/E7 mRNA检测联合宫颈活检对宫颈上皮内瘤变和宫颈癌的筛查有临床意义。 Objective: To research the diagnosis value by determining high risk human papilloma virus(HPV) E6/E7 mRNA combined with cervical biopsy in the sample with cervical intraepithelial neoplasia after detected by liquid based thin-prep cytology test(TCT). Methods: The high risk HPV E6/E7 mRNA was detected in 100 TCT samples with different degrees of cervical lesions. The cervical biopsy examination was done for all the patients with positive or negative of HPV E6/E7 mRNA test. The correlation between the expression of HPV E6/E7 mRNA and the cytological grades was analyzed. Results: The copy number of HPV E6/E7 mRNA was statistically different in various cytological grades (P〈0.05). The copy number of HPV E6/E7 mRNA was correlated with severe degree of epithelial cervical dysplasia. The copy number of HPV E6/E7 mRNA was positively correlated with positive rate of cervical biopsy. Conclusions: High risk HPV E6/E7 mRNA test combined with cervical biopsy is of clinical value for diagnosis of cervical intraepithelial neoplasia.
出处 《生殖医学杂志》 CAS 2013年第7期514-516,共3页 Journal of Reproductive Medicine
关键词 人乳头瘤病毒 mRNA 新柏超薄液基细胞学检查 E6 E7 宫颈活检 Human papilloma virus mRNA TCT E6/E7 Cervical biopsy
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参考文献5

  • 1Lie AK, Kristensen G. Human papillomavirus E6/E7 mRNA testing as a predictive marker for cervical cancinoma [J ]. Expert Revol Diagn, 2008,8:405-415.
  • 2Cuschieri K, Wentzensen N. Human papiloma virus mRNA and p16 detection as biomarkers for the improved diagnosis of cervical neoplasia[J]. Cancer Epideminol Biornarkers Prey, 2008,17 : 2536-2545.
  • 3Cattani P,Siddu A,DOnghia S,et al. RNA(E6 and E7)assays versus DNA(E6 and E7)assays for risk evaluation for women infected with human papilloma virus [J]. J Clin Microhiol, 2009,47 : 2136-2141.
  • 4Galarowicz B,Jach R, Kidzierska J, et al. The role of mRNA E6/E7 HPV high oncogenic risk expression in colposcopy of cervical intraepithelial neoplasia(CIN) [J]. Przegl Lek,2012, 69:651-657.
  • 5金滢,潘凌亚,王友芳,程雪梅,郎景和.高危型人乳头瘤病毒检测在宫颈细胞学异常患者中的分流作用[J].中华医学杂志,2008,88(17):1173-1176. 被引量:10

二级参考文献9

  • 1Walboomers JM, Jacobs MV, Manos MM, et al. Human papiUomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol, 1999,189 : 12-19.
  • 2Saslow D, Runowicz CD, Solomon D, et al. American Cancer Society. American Cancer Society guideline for the early detection of cervical neoplasia and cancer. CA Cancer J Clin, 2002,52: 342-362.
  • 3American College of Obstetricians and Gynecologists. ACOG practice bulletin. Cervical Cytology screening. Number 45, August 2003, Int J Gynaecol Obstet, 2003,83:237-247.
  • 4Donna Dehn, Kathleen C. Torkko, Kenneth R. Shroyer. Human papiUomavirus testing and molecular markers of cervical dysplasia and carcinoma. Cancer, 2007,111:1-7.
  • 5Solomon D, Schiffman MH, Tarene R. Comparison of three management strategies for patients with atypical squamous cells of undetermined significance: baseline results from a randomized triaL J Natl Cancer Inst, 2001,93:293-299.
  • 6ASCUS-LSIL Traige Study (ALTS) Group. A randomized trial on the management of low-grade squamous intraepithelial lesion cytology interpretations. Am J Obstet Gynecol, 2003, 188: 1393-1400.
  • 7Thomas Cox JT. The Clinician's view: role of human papillomavirus testing in the American society for colposcopy and cervical pathology guidelines for the management of abnormal cervical cytology and cervical cancer precursors. Arch Pathol Lab Med, 2003,127 : 950-958.
  • 8Wright TC Jr, Cox JT, Massad LS, et al. 2001 Consensus guidelines for the management of women with cervical cytological abnormalities. JAMA, 2002,287:2120-2129.
  • 9Wensveen CW, Kagie M J, Nagelkerke N J, et al. Can viral load, semi-quantitatively evaluated, of human papiUomavirus predict cytological or histological outcome in women with atypical squamous or glandular cells of undetermined significance cytology? Eur J Gynaecol Oncol, 2005,26:393-397.

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