摘要
目的回顾性分析丙型肝炎患者检验结果,探讨丙型肝炎的有效检验方法。方法对209份标本进行酶联免疫法(ELISA)检测抗-HCV和实时荧光定量PCR法(FQ-PCR)检测HCV-RNA,分析两种检测方法的有效性及差异。结果检出抗-HCV阳性138份(66.03%),HCV-RNA阳性128份(61.24%),差异无统计学意义(P>0.05)。抗-HCV与HCV-RNA均阳性者112份(53.59%),两者均阴性的55例(26.32%)。两种检测方法总符合率为79.90%(配对x2=2.3810,P>0.05)。结论 ELISA和FQ-PCR法检测HCV比较无显著差异,但二者结合检测可提高HCV检出率。
Objective To retrospectively analyze the test results of patients with hepatitis C and explore the effective inspection method for hepatitis C. Methods Anti-HCV was detected by enzyme-linked immunoassay（ELISA） and HCV-RNA was detected by real-time fluorescence quantitative PCR（FQ-PCR） in 209 specimens to analyze the effectivenesses and differences of the two methods. Results 138（66.03%） cases were detected positive for anti- HCV,while 128（61.24%） cases were detected positive for HCV-RNA（P 〉 0.05）. 112 cases （53.59%） were positive for both Anti-HCV and HCV-RNA,while 55 cases （26.32%） were negative for both Anti-HCV and HCV-RNA.The total coincidence rate of the two methods was 79.90%（paired χ2=2.3810,P 〉 0.05）. Conclusion There is no significant difference of detecting HCV between ELISA and FQ-PCR,however,the detection rate of HCV can be improved by combination of the two methods.
出处
《中国医药科学》
2013年第13期125-126,共2页
China Medicine And Pharmacy
关键词
丙型肝炎
检验
实时荧光定量PCR法
酶联免疫法
Hepatitis C
Detection
Real-time fluorescent quantitative PCR method
Enzyme-linked immunoassay
