摘要
目的:探讨维生素D受体(VDR)TaqI位点基因多态性与甘肃东乡族及保安族人群龋病易感性的关系。方法:对甘肃东乡族及保安族龋病患者264人和健康对照219人采集外周静脉血,提取DNA,利用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法,测定VDR TaqI位点的基因型分布及基因频率,用SPSS16.0软件包进行数据分析。结果:以TT为参照,Tt基因型频率病例组(14.0%)显著高于对照组(4.3%)(P=0.000 2);个体携带基因型Tt较TT患龋风险提高了3.8倍,等位基因t在病例组占7.0%,在对照组占2.1%(P=0.000 3);按不同性别及族群进行多态性分析,差别无统计学意义。结论:等位基因t可能与该人群龋病易感性有关。
Objective：To investigate the relationship between the gene polymorphisms of vitamin D receptor（VDR）TaqI locus and dental caries susceptibility in Dongxiang and Bao＇an minorities in Gansu province.Methods： Total DNA samples were extracted from peripheral venous blood from 264 Dongxiang and Bao＇an subjects with carious and 216 healthy controls.The distribution of the genotypes of TaqI locus and allele frequency were examined by polymerase chain reaction-restriction fragment length polymorphism assay（PCR-RFLP）.The genotype TT was used as the reference,the data were statistically analyzed by the SPSS 16.0 software package.Results： The frequency of the genotype Tt in the case group（14%） was significantly higher than that in the control group（4.3%）（P=0.000 2）.The risk of dental caries in the individuals with Tt was increased by 3.8 times than that in the individuals with TT.The proportion of allele t in the case group was 7.0% and that in the control group 2.1%（P=0.000 3）.According to the different genders and nationalities,the results were analyzed by using the method of polymorphism analysis,the differences were not of statistical significance.Conclusion： The allele t might be relevant to dental caries susceptibility of Dongxiang and Bao＇an people.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2013年第4期535-538,共4页
Journal of Practical Stomatology
基金
甘肃省国际科技合作项目(编号:2009GS03605)
关键词
维生素D受体
多态性
龋病
病例对照研究
Vitamin D receptor
Polymorphism
Dental caries
Case-control study
