摘要
目的探讨氟化钠(NaF)对人成骨肉瘤MG-63细胞增殖的影响。方法以0、10-2、10-1、1、10、102、5×102、103、5×103、104、2×104、4×104μmol/L NaF染毒MG-63细胞,培养48 h。采用MTT实验观察NaF对MG-63细胞增殖能力的影响,以流式细胞技术检测MG-63细胞周期变化。结果 MTT实验结果显示,MG-63细胞开始有增殖活性时的NaF浓度为102μmol/L,增殖活性最大时的浓度为5×103μmol/L,增殖活性受到抑制时的浓度为2×104μmol/L。流式细胞技术检测结果显示,采用MTT实验中确定的3个关键浓度染毒细胞48 h后,高剂量(2×104μmol/L)组的MG-63细胞凋亡率最高(6.001%),细胞增殖指数(PI)最低(0.339);中剂量(5×103μmol/L)组的细胞凋亡率最低(2.220%),PI最高(0.632);低剂量(102μmol/L)组的PI及细胞凋亡率均与对照组无明显差异。结论 NaF对成骨细胞具有双向作用,低浓度下促进细胞增殖,超过一定浓度后因其增殖抑制而使细胞凋亡增加。
Objective To explore the effects of sodium fluoride exposure on proliferation of human osteosarcoma MG- 63cells. Methods MG-63 cells were exposed to 0,10-2,10-1, 1,10,102,5×102, 103,5×103, 104,2×104 and 4×104 μmol/L NaF solution for 48 h. MTT assay was used to measure proliferation of MG-63 cells, and flow cytometry was applied to test the ceil cycles. Results At the concentration of 102 μmol/L NaF, MG-63 cells proliferation became increased; At 5×103 μmol/L NaF, cells proliferation rate reached the peak, but at 2×104 μmol/L, ceils proliferation became inhibited. The three concentrations would be taken as the low-dose, moderate-dose and high-dose in the fallowing experiments. After treatment by sodium fluoride for 48 h, cells in high-dose group had the highest apoptosis rate and the lowest PI （P〈0.05）, while cells in moderate-dose group had the lowest apoptosis rate and the highest PI （P〈0.05）. In term of the tow-dose and control group, there was no significant difference in those two parameters. Conclusion Sodium fluoride has a double action to osteoblast. NaF can promote proliferation at lower concentration and inhibit proliferation at higher concentration. The double action may be related to the multiple types of pathological manifestations of skeletal fluorosis.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2013年第7期597-599,共3页
Journal of Environment and Health
基金
贵州省科学技术基金(黔科合J字[2010]2268号)
贵阳医学院院基金(k2009-3)
