摘要
目的观察微生态制剂贝飞达(双歧杆菌三联活菌肠溶胶囊)治疗高脂饮食所致大鼠非酒精性脂肪肝的疗效并探讨其可能的作用机制。方法雄性SD大鼠32只,适应性饲养1周后,随机分为3组,正常组:12只给予普通饲料喂养;模型组:12只,贝飞达治疗组:8只,均给予高脂饲料喂养;于喂养12周末正常组及模型组各处死4只,经肝组织HE染色确定造模成功后,贝飞达治疗组给予贝飞达[0.113 g/(kg.d)]灌胃,于16周末全部处死。检测大鼠血清AST、ALT、TC、TG、LDL、HDL、血清内毒素水平,观察其肝组织学变化。结果模型组于高脂饲料喂养12周末出现脂肪肝,与正常组比较,模型组大鼠血清AST、ALT、TG、TC、LDL、HDL及血清内毒素水平均明显升高(P<0.01)。贝飞达治疗组大鼠各项指标较模型组均有显著改善,肝脏脂肪变性程度减轻。结论微生态制剂贝飞达可能通过改善肠道菌群紊乱,减轻内毒素血症,从而调节肝脏脂质代谢紊乱,对非酒精性单纯性脂肪肝起到治疗作用。
Objective To explore the therapeutic effect and mechanisms of Bei Fei Da（enteric-coated Bifid-triple Viable Capsules） in the treatment of nonalcoholic fatty liver disease（NAFLD） in rats fed with high-fat diets.Methods 32 male Sprague Dawley rats were randomized into 3 groups after fed with normal diet for 1 week.The control group（n=12） was fed with normal diets.The model group（n=12） and the therapy group（n=8） were fed with high-fat diets（consisting of 88% of normal diet ＋ 10% of lard ＋ 1% of cholesterol ＋ 5% of egg yolk powder）.4 Rats in control and 4 in model groups were killed at Week 12,and pathological changes were observed by HE stain to make sure the model was built successfully.The therapy group was treated with enteric-coated Bifid-triple viable Capsules [0.113 g/（kg·d）].All the rats were executed at the end of Week 16.Blood samples were collected for detection of serum aspartate transaminase（AST）,alanine aminotransferase（ALT）,total cholesterol（TC）,thermogravimetry（TG）,low density lipoprotein（LDL）,high density lipoprotein（HDL） and endotoxin.The histological changes of liver tissues were observed by HE stain.ResultsThe rats in the model group developed nonalcoholic fatty liver disease after 12 weeks.Compared with normal control group,the levels of AST,ALT,TC,TG,LDL,HDL and endotoxin in the serum increased in the model group（P〈0.01）,while those in the therapy group were improved obviously.Conclusion Enteric Bifid-Triple Viable Capsules have a therapeutic effect on NAFLD,possibly by regulating gut microbiota,reducing endotoxin and regulating lipid metabolism.
出处
《中国微生态学杂志》
CAS
CSCD
2013年第7期793-796,共4页
Chinese Journal of Microecology
