摘要
目的:建立磷钼钨酸/干酪素分光光度法测定不同产地藏药余甘子中总鞣质含量的方法。方法:以没食子酸为对照品,磷钼钨酸为显色剂,检测波长760 nm,测定8个不同产地余甘子中总鞣质含量。结果:没食子酸在0.306～2.448 mg.L-1与吸光度呈良好线性关系(r=0.999 1),平均加样回收率99.27%,RSD 2.30%。不同产地余甘子药材总鞣质含量差别较大(6.72%～11.95%),印度和尼泊尔产余甘子中总鞣质含量最高。结论:该方法简单、准确、重复性好,可用于测定余甘子中总鞣质含量。
Objective： To develop a phosphomolybdic acid-casein spectrophotometry for determination of total tannins in fruits of Phyllanthus emblica from different areas.Method： With gallic acid as reference substance and phosphomolybdic acid as chromogenic agent,detection wavelength 760 nm,the content of total tannins in P.emblica from eight different areas was determined.Result： The linearity range of gallic acid was 0.306-2.448 mg.L-1 with r = 0.999 1,the average recovery was 99.27% with RSD 2.30%.The content of total tannins in P.emblica from different areas were significantly different（6.72%-11.95%）,the content of total tannins in samples from India and Nepal were the highest.Conclusion： This method was simple,accurate and reproducible which could be adopted for determination of total tannins in P.emblica.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第15期61-63,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81274187)
北京中医药大学自主课题(2009TYB22JS027)
北京中医药大学创新团队项目(2011-CXTD-12)
关键词
余甘子
总鞣质含量
不同产地
没食子酸
Phyllanthus emblica
the content of total tannins
different locations
gallic acid
