摘要
目的:探讨苦参碱对宫颈癌Hela细胞增殖、凋亡的影响及其可能的分子机制。方法:应用不同浓度的苦参碱作用于人宫颈癌Hela细胞,采用MTT法检测细胞增殖;Annexin V FITC/PI双染色流式细胞仪检测细胞凋亡和细胞周期;RT-PCR和Western blotting分析Survivin基因表达。结果:苦参碱对Hela细胞的体外增殖具有抑制作用,量效关系显著,与对照组比较有统计学差异(P<0.01),半数抑制浓度(IC50)为2.60 g.L-1。经流式细胞仪检测表明,苦参碱能使Hela细胞G0-G1期逐渐增加,G2-M期和S期逐渐减少,并且随着剂量的增加,Hela细胞凋亡率明显增加(P<0.01)。苦参碱对Hela细胞Survivin基因表达有一定的抑制作用(P<0.01),并呈剂量依赖性。结论:苦参碱能有效抑制人宫颈癌Hela细胞的增殖,诱导其凋亡,其作用机制可能与抑制Survivin基因的表达有关。
Objective： To investigate the effects of matrine on proliferation,apoptosis in human cervical cancer Hela cells and its possible molecular mechanism.Method： Hela cells were treated with matrine of different concentrations.MTT assay was used to measure the cell proliferative effect.The Annexin V FITC / PI double staining analysis by flow cytometry was used to evaluate apoptotic rate and the cell cycle.RT-PCR and western blotting were performed to detect the expression of Survivin gene.Result： Matrine could inhibit the proliferation of Hela cells in vitro,and dose-effect relationship was significant（P 0.01）.IC50 of Hela cells was 2.60 g.L-1.The flow cytometry test indicated that matrine could make Hela cells G0-G1 phase gradually increasing,G2-M period and S phase reduce gradually,and with the increase of the dose,Hela cells apoptosis rate increased significantly（P 0.01）.Matrine could suppress the expression of Survivin gene（P 0.01）,and with the dose-dependent manner.Conclusion： Matrine could effectively inhibit proliferation and induce apoptosis of Hela cells.Its molecular mechanisms might be related to modulating the expression of Survivin.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第15期235-238,共4页
Chinese Journal of Experimental Traditional Medical Formulae
