摘要
采用菌丝生长速率法测定了新化合物氟醚菌酰胺(LH-2010A)对10种常见植物病原菌的毒力,同时研究了其对棉花立枯丝核菌无性繁殖过程、菌丝形态及细胞膜通透性的影响。结果表明:氟醚菌酰胺对10种病原菌均具有较强的抑菌活性,比结构相似的选择性杀菌剂氟吡菌胺具有更广的杀菌谱。氟醚菌酰胺对棉花立枯丝核菌和番茄灰霉病菌的抑菌活性最高,EC50值均小于1.00μg/mL,对其他8种病原菌的EC50值则在1.10～4.63μg/mL之间;而氟吡菌胺只对辣椒疫霉和马铃薯晚疫病菌具有较高的抑菌活性,EC50值分别为0.61和0.72μg/mL。在100.0μg/mL下,氟醚菌酰胺对棉花立枯丝核菌抑菌作用明显,能显著抑制其菌核产生和菌丝生长量,使菌丝分支增多、变短、变粗;以不同浓度的氟醚菌酰胺处理棉花立枯丝核菌时,其细胞膜的通透性随着药剂浓度的增加而显著增大,520 min时最高处理浓度下的相对渗率为77.01%。表明氟醚菌酰胺对多种植物病原菌具有较高的抑菌活性,并能明显抑制棉花立枯丝核菌的多项生理指标。
The mycelial linear growth rate method was adopted to determine the toxicity of LH-2010A to 10 pathogens, and the effects of LH-2010A on the process of asexual reproduction, membrane permeability and mycelial morphology of Rhizoctonia solani were also detected. The results indicated that LH-2010A had broader fungicidal spectrum than another formamide fungicide, fluopicolide with effective activity against 10 pathogens. The highest toxicity of LH-2010A to R. solani and Botrytis cinerea were obtained with EC50value less than 1.00 μg/mL,while the eight others with EC50 1.10 -4. 63 μg/mL. However, fluopicolide only exhibited high toxicity to Phytophthora capsici and Phytophthora infestans with ECso values as 0.61 μg/mL and 0.72 μg/mL, respectively. In addition, LH-2010A had high fungicidal activity against sclerotinia and mycelial growth of R. solani, and it only showed inhibitory effect on R. solani at concentration less than 100.0 mg/L. Moreover,the amount of ramification increased and the mycelium formed irregular ramification or abnormal configuration （contracted or swelled up ） on mycelium tip. With the increase of LH-2010A concentration, the membrane permeability of R. solani increased, and the highest relative leakage reached 77.01% at 520 rain. In conclution, LH-2010A has high fungicidal activity against various pathogens and it can inhibit some physiological characteristics of R. solani.
出处
《农药学学报》
CAS
CSCD
北大核心
2013年第4期405-411,共7页
Chinese Journal of Pesticide Science
基金
"十二五"国家科技支撑计划(2011BAE06B02)
公益性行业(农业)科研专项(201003004)
