摘要
【目的】了解薏米多酚的体内抗氧化能力,着重分析薏米结合多酚的抗氧化能力,确定结合多酚对总抗氧化能力的贡献;研究薏米多酚对肝癌细胞的毒性和抗增殖作用,在细胞层面上对薏米多酚的功能作用进行评价分析。【方法】以贵州黑谷薏米、辽宁5号薏米、龙薏1号薏米为原料,通过对薏米样品多酚类物质游离型和结合型部分构建细胞模型,评价细胞内抗氧化能力指数,以HepG2细胞为研究对象,测定分析薏米多酚提取物对细胞毒性的中位浓度CC50值和细胞抗增殖作用的半最大效应浓度EC50值。【结果】薏米结合型多酚抗氧化能力显著高于游离型多酚。不同品种间总多酚含量和抗氧化能力差异显著,辽宁5号薏米和龙薏1号薏米明显优于贵州黑谷薏米。对于HepG2细胞,薏米品种对于人体肝癌细胞具有明显的增殖抑制作用,且基本在试验浓度范围内,薏米样品本身无细胞毒性的存在。【结论】薏米作为中国传统的药食两用全谷物食品,含有丰富的多酚类物质,具有较强的抗氧化能力,之前的研究,往往仅考虑薏米游离型多酚,实际薏米中还存在大量结合型多酚。考虑到此结合型多酚,薏米多酚的抗氧化能力明显提升,且薏米多酚对肿瘤细胞具有较明显的抗增殖作用。
[ Objective] The objective of this study is to learn and analyze the antioxidant capacity of adlay extracts, especially that of the bound part , and to determine the contribution of the bound part to the total antioxidant capacity. The toxicity and aati-proliferative effect of adlay polyphenols on liver cancer cells were also studied. In the meanwhile, the functions of adlay extracts were evaluated and analyzed at cell level. [ Method ] Guizhou heigu adlay, Liaoning 5 adlay and Longyi 1 adlay were used as raw materials, the intracellular ORAC was evaluated by building cell models of free or bound polyphenols of adlay extracts. With human hepatoma cells HepG2 as the research object, CCs0 for cytotoxicity and ECs0 for anti-proliferative effect were determined and analyzed. [ Result ] The antioxidant capacity of bound polyphenol is significantly higher than that of the free part. The content of totel polyphenol and the antioxidant capacity are obviously different among different species. Liaoning 5 adlay and Longyi 1 adlay are significantly better than Guizhou Heigu adlay. The adlay extracts have an obvious effect on inhibition of proliferation of human liver cancer cells, and substantially in the experimental concentration range, the adlay sample itself has no cytotoxicity. [ Conclusion ] As a traditional Chinese medical and edible whole grain foods, adlay has abundant polyphenols and strong antioxidant capacity. The antioxidant value significantly improved after considering the existence of bound polyphenols. Besides, polyphenol extracts of adlay have an obvious anti-proliferative effects on tumor cells.
出处
《中国农业科学》
CAS
CSCD
北大核心
2013年第14期2990-3002,共13页
Scientia Agricultura Sinica
基金
江苏省科技支撑计划项目(BE2012764)
国家"863"计划项目(2013AA102207-2)
国家科技支撑计划项目(2012BAD33B04)
关键词
薏米
多酚
CAA
毒性
抗增殖
adlay
polyphenols
CAA
cytotoxicity
anti-proliferation
