摘要
目的:检测妊娠期肝内胆汁淤积症(ICP)产妇脂质过氧化物及雌激素水平改变,借以探讨其相关的临床意义。方法:收集该院产科2008年12月～2012年12月住院分娩的妊娠期肝内胆汁淤积症64例,另取该院同期分娩的健康产妇40例作为对照,ICP孕妇分为轻度组(n=33)和重度组(n=31)。超氧化物歧化酶(SOD)和丙二醛(MDA)的检测采用分光分析法,雌二醇(E2)测定使用ELISA法。结果:对照组中SOD、MDA和E2表达水平分别为(204.41±26.57)U/ml、(16.43±4.22)nmol/ml和(157.15±26.44)μg/ml,ICP组中分别为(212.37±27.41)U/ml、(34.63±7.15)nmol/ml和(232.72±36.52)μg/ml,统计分析显示SOD在两组之间不具有显著性差异(P>0.05),而MDA和E2在ICP组显著高于对照组(P<0.01)。轻度组中SOD、MDA和E2表达水平分别为(211.26±25.31)U/ml、(28.54±5.03)nmol/ml和(201.62±30.73)μg/ml,重度组中分别为(213.72±28.24)U/ml、(41.11.63±7.84)nmol/ml和(265.83±41.84)μg/ml,统计分析显示SOD在两组之间不具有统计学差异(P>0.05),而MDA和E2在轻度组显著低于重度组(P<0.05)。结论:妊娠期肝内胆汁淤积症产妇体内存在MDA和E2的异常高表达,高的脂质过氧化状态和雌激素水平可能参与了妊娠肝内胆汁淤积症的发生、发展过程。
Objective： To investigate lipid perioxidation and the expresssion of estriol in intrahepatic cholestasis of pregnancy （ICP） and explore its clinical significance. Methods： 64 pregnant women with ICP were enrolled in our study. Another 40 normal pregnant women were collected as normal control. 64 cases of ICP pregnant women were divided into two groups ： mild （ n = 33 ） and severe groups （ n = 31 ） . The expression of superoxide dismutase （SOD） and malonodialdehyde （MDA） were detected by spectrometry analysis. The ex-pression of estradiol （ E2 ） was detected by ELISA analysis. Results ： The expression of SOD, MDA, and E2 in control group were （204.41 ±26.57） U/ml, （16.43 ±4.22） nmol/ml, and （157.15 ±26.44） μg/ml, and in ICP groups were （212.37±27.41） U/ml, （34. 63 ±7.15） nmol/ml, and （232. 72 ± 36. 52） μg/ml. There was no significant difference in SOD expression between the two groups （ P 〉 0. 05 ） . However, the expressions of MDA and E2 in ICP groups were higher than those of control group （ P 〈 0. 01 ） . The expressions of SOD, MDA, and E2 in mild group were （ 211.26 ± 25.31 ） U/ml, （28.54 ± 5.03 ） nmol/ml, and （201.62 ± 30.73） Ixg/ml, and in severe groups was （213.72 -±28.24 ） U/ml, （41.11.63 ±7.84） nmol/ml, and （265.83 -±41.84） μg/ml. There was no significant difference in SOD expression between the two groups （ P 〉 0. 05 ） . However, the expressions of MDA and E2 in severe groups were higher than those of mild group （ P 〈 0.05 ） . Conclusion： The overexpressions of MDA and E2 are found in ICP pregnant women. The imbalance of lipid perioxidation and strong expression of estriol may be involved in the occurrence and development of ICP.
出处
《中国妇幼保健》
CAS
北大核心
2013年第23期3758-3760,共3页
Maternal and Child Health Care of China
关键词
妊娠期肝内胆汁淤积症
超氧化物歧化酶
丙二醛
雌二醇
Intrahepatic cholestasis of pregnancy
Superoxide dismutase
Malonodialdehyde
Estradiol
