摘要
目的观察低剂量砷染毒致小鼠肝脏和血清肝功能及氧化损伤情况。方法 40只健康KM小鼠随机分组,设染毒砷剂量为0.0(对照组)、12.5(低剂量组)、25.0(中剂量组)、50.0(高剂量组)μg/L 4组,雌雄各半,经饮水途径染毒,染毒时间为10周,检测小鼠肝脏和血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平。结果血清中,高剂量组AST与对照组比较显著增高,差异有统计学意义(P<0.05);GSH-Px活性与对照组相比,GSH-Px活性有明显改变,而中、高剂量组无明显变化。肝组织中,中剂量组清蛋白水平显著增高(P<0.05)。结论低砷可以诱导小鼠肝功能损伤,但是抗氧化酶GSH-Px活性在染毒组则增高,对抗其氧化损伤作用。
Objective To observe the effects on dimethylformamide liver function and oxidative stress of low level arsenic on mouse liver and serum.Methods 40health KM mouses were randomly divided into 0,12.5,25.0,50.0μg/L arsenic and exposured 10weeks.The ALT,AST,MDA,SOD,GSH-Px in serum and liver were determined.Results In serum：the AST was significantly increase in 50.0μg/L dose（P0.05）;GSH-Px were significant increase in 12.5μg/L dose（P0.05）.In the liver：the albumin was significantly increase in middle dose（P0.05）.Conclusion Low arsenic can induce liver damage on mice,but can increase antioxidatant GSH-Px in arsenic group.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第23期2758-2759,共2页
Chongqing medicine
基金
赣南医学院人才引进资助项目(2009052)
